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具有不同Tn3移动遗传元素的广泛耐药分离物的分子特征和遗传分析

Jiazhen Wang¹, Xin Dong², Fengming Wang^1,2

¹School of Public Health, Xuzhou Medical University, Xuzhou, 221004, China.

Current microbiology

|June 19, 2023

概括

广泛耐药 (XDR) 细菌构成了重大威胁. 这项研究跟踪了州医院的XDR Klebsiella pneumoniae,Acinetobacter baumannii和Escherichia coli,确定了关键的耐药性基因和新型基因型,以指导感染控制.

科学领域:

医学微生物学医学微生物学
传染性疾病传染性疾病
分子流行病学分子流行病学

背景情况:

广泛耐药 (XDR) 细菌是一个日益严重的全球健康问题,导致难以治疗的临床感染.
了解XDR病原体的分子流行病学对于有效的感染控制策略至关重要.

研究的目的:

调查来自长州医院的XDR Klebsiella pneumoniae,Acinetobacter baumannii和Escherichia coli分离物的分子流行病学.
为了确定这些XDR细菌群体中普遍存在的抗生素耐药性基因和遗传特征.

主要方法:

抗生素敏感性测试和表型分析.
多部位序列类型 (MLST) 和脉冲场凝电泳 (PFGE) 用于菌株特征.
抗性基因和等离子体的测序.

主要成果:

确定了包括TEM,CTX-M,OXA-48和KPC在内的多种抗性基因.
曼尼菌株属于ST224,携带 bla-CTX-M-2/TEM. 一个新的K. pneumoniae基因型,ST2639,被发现.
在2.3%的菌株中发现了bla_NDM-1或bla_NDM-5基因,通常与Tn3相关的移动遗传元素和ISKox3.3相关.

结论:

抗生素耐药性基因在医院病房中分布不均,凸显了针对性干预的必要性.
识别与耐药性相关的新型基因型和移动遗传元素,特别是产生NDM的菌株,对于管理XDR感染至关重要.
追踪抗生素耐药性的来源,特别是MBL编码基因,对于控制XDR细菌的传播至关重要.

关键词:

碳烯的质量也很大.具有广泛的耐药性.分子表征的分子表征.有关Tn3的移动基因元素.

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