通过Aureobasidium Melanogenum使用简化介质和pH控制的发酵策略与细胞形态分析有效生产黑色素
Ran Zhou1,2, Lan Ma2, Xuwen Qin2
1Key Laboratory of Biochemistry and Molecular Biology (Guilin Medical University), Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, 1 Zhiyuan Road, Guilin, 541199, Guangxi, China.
Applied biochemistry and biotechnology
|June 19, 2023
概括
黑酵母发酵有效地产生黑色胺,一种具有多种应用的生物聚合物. 优化的策略显著提高了黑色素产量,达到18.50g/L,为工业生产铺平了道路.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 生物聚合物生产 生物聚合物生产
背景情况:
- 天然黑色素是一种多功能生物聚合物,在医学,化品和环境保护等领域都有应用.
- 微生物发酵提供了一种有效的黑色素生产途径.
- 黑色酵母Aureobasidium melanogenum因其黑色蛋白分泌能力而受到研究.
研究的目的:
- 使用Aureobasidium melanogenum开发一个优化的发酵策略,以提高黑色素的产量.
- 研究细胞形态和黑色素合成之间的关系.
- 为了描述产生的黑色素.
主要方法:
- 一种简单的寡质基质介质被设计用于黑色素的生产.
- 发酵在5L发酵器中进行,使用各种策略,包括pH控制,营养添加和过氧化刺激.
- 分析了细胞形态,以与黑色素合成相关联.
- 产生的黑色素因其结构性质而具有特点.
主要成果:
- 在没有pH控制的简单介质中,达到6.64g/L的黑色素标位.
- 克拉米多形态被确定为最佳的黑色素合成.
- 一个优化的发酵策略产生了18.50g/L的最大黑色素标位,增加了178.6%.
- 生产的黑色素被识别为具有体结构的eumelanin.
结论:
- 一种简单的,寡质的介质可以诱导Aureobasidium melanogenum中的黑色素产生.
- 优化的发酵策略,包括pH值控制和营养素/兴奋剂添加,显著提高黑色素产量.
- 这项研究提出了一种可行的方法,用于工业规模生产eumelanin.
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