绿色的一步合成从Schinus areira树叶提取物中获得的银纳米颗粒:特征和抗菌机制分析
Anike P V Ferreyra Maillard1, Anahí Bordón1,2, Andrea C Cutro1,3
1Laboratorio de Compuestos Bioactivos, Centro de Investigación en Biofísica Aplicada y Alimentos (CIBAAL), CONICET, Universidad Nacional de Santiago del Estero, RN 9 Km 1125, 4206, Santiago del Estero, Argentina.
Applied biochemistry and biotechnology
|June 19, 2023
概括
绿色合成的银纳米粒子 (AgNPs) 来自Schinus areira叶提取物表现出强大的抗菌活性. 这些AgNP有效地通过诱导氧化应激和破坏细胞膜来对抗抗生素耐药细菌.
科学领域:
- 纳米技术纳米技术
- 微生物学 微生物学
- 绿色化学 绿色化学
背景情况:
- 抗生素耐药细菌对全球健康构成重大威胁.
- 银纳米颗粒 (AgNPs) 显示出作为抗菌剂的前景.
- 开发AgNP合成的可持续方法至关重要.
研究的目的:
- 为了合成银纳米粒子 (AgNPs) 使用绿色协议与Schinus areira叶提取物.
- 描述合成的AgNP并评估它们的抗微生物疗效.
- 阐明AgNP介导的细菌死亡背后的机制.
主要方法:
- 绿色合成AgNP使用水性Schinus areira树叶提取物.
- 使用UV-Vis光谱,DLS,TEM和拉曼光谱对AgNP进行表征.
- 确定最小抑制度 (MIC) 和最小杀菌度 (MBC) 针对金黄色葡萄球菌和大肠杆菌.
- 评估细胞内活性氧物种 (ROS) 水平和细菌膜完整性.
主要成果:
- 成功合成了直径约为11纳米,表面电荷为负的近球形AgNP.
- AgNPs对黄金葡萄球菌 (格拉姆阳性) 和大肠杆菌 (格拉姆阴性) 均表现出显著的抗菌活性.
- 在两种细菌物种中,AgNP诱导了细胞内ROS的产生,并在大肠杆菌中引起了膜损伤.
结论:
- 树叶提取物促进了有效的抗微生物AgNP的稳定,绿色合成.
- 通过ROS诱导和膜破坏,AgNP表现出广泛的抗菌活性.
- 这些发现支持AgNP作为传统抗生素可行的替代品的潜力.
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