植物细胞核的染色素免疫染色
Nobuko Ohmido1, Aqwin Polosoro2,3
1Graduate School of Human Development and Environment, Kobe University, Kobe, Japan. ohmido@kobe-u.ac.jp.
Methods in molecular biology (Clifton, N.J.)
|June 19, 2023
概括
这项研究详细介绍了可视化根表观遗传变化的方法,揭示了盐度和生长激素等环境因素如何通过染色质修饰影响植物发育.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 表观遗传修饰,如基因组和DNA甲基化,对于调节植物生长和发育至关重要.
- 免疫覆盖技术允许可视化植物组织中的特定染色质修饰.
研究的目的:
- 描述实验程序,以确定米根染色蛋白中的素H3甲基化模式 (H3K4me2,H3K9me2).
- 分析铁和盐分压力对表观遗传景观的影响,使用异色素 (H3K9me2) 和欧色素 (H3K4me) 标记物.
- 调查环境压力和植物生长调节剂 (盐度,auxin,酸) 对大米根发育的表观遗传影响.
主要方法:
- 开发免疫染协议,以检测H3K4me2和H3K9me2模式在3D整根组织和2D单核中.
- 盐度,铁,奥克和酸处理对米植物的应用.
- 在大米根的近端美里斯蒂姆区域的染色质修饰的分析.
主要成果:
- 已确立的方法可用于可视化米根染色蛋白中的特定的组素甲基化模式.
- 根据盐度压力和植物生长调节者的反应,表观遗传景观发生了明显的变化.
- 鉴定了与异性染色素和欧性染色素标记物相关的独特表观遗传特征.
结论:
- 描述的方法提供了一个强大的方法来研究大米根的动态表观遗传景观.
- 环境压力和生长调节剂对大米根表观遗传有很大影响,影响了生长和发育.
- 了解这些表观遗传变化是提高作物弹性和生产率的关键.
相关概念视频
01:01Euchromatin
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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
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02:36Chromatin Immunoprecipitation- ChIP
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Types of ChIP
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01:32Chromatin Modification in iPS Cells
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02:35Chromatin Position Affects Gene Expression
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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
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02:38Heterochromatin
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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
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01:32Chromatin Packaging
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Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
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