Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Author Correction: Allelic variation at a single locus distinguishes spring and winter faba beans.

Nature genetics·2026
Same author

A chromosome-scale genome assembly of Hordeum erectifolium: genomic, transcriptomic and anatomical adaptations to drought in a wild barley relative.

The New phytologist·2026
Same author

Allelic variation at a single locus distinguishes spring and winter faba beans.

Nature genetics·2026
Same author

Epigenome and interactome profiling uncovers principles of distal regulation in the barley genome.

Cell genomics·2025
Same author

The maize B chromosome shapes the transcriptome throughout the entire plant life cycle.

Journal of experimental botany·2025
Same author

A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum.

Nature·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jul 26, 2025

High-Throughput Analysis of Optical Mapping Data Using ElectroMap
07:36

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

Published on: June 4, 2019

9.4K

流量排序辅助的光学映射.

Hana Šimková1, Zuzana Tulpová2, Petr Cápal2

  • 1Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, Olomouc, Czech Republic. simkovah@ueb.cas.cz.

Methods in molecular biology (Clifton, N.J.)
|June 19, 2023
PubMed
概括
此摘要是机器生成的。

光学映射需要超长的高分子量DNA (uHMW DNA). 流式细胞测量有效地净化植物核,用于HMW DNA隔离,从而实现基因组和染色体光学图.

关键词:
生物纳米基因组地图染色体是一种染色体.流细胞计和分类流细胞计.在HMWDNA制备中使用HMWDNA制备原子核中的原子核.光学映射绘制的光学映射.超长的高分子量DNA.

更多相关视频

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K
Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

9.6K

相关实验视频

Last Updated: Jul 26, 2025

High-Throughput Analysis of Optical Mapping Data Using ElectroMap
07:36

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

Published on: June 4, 2019

9.4K
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K
Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

9.6K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 光学映射对于基因组研究至关重要,有助于序列组装和结构变异分析.
  • 从植物中获得超长的高分子量DNA (uHMW DNA) 是很困难的,因为细胞壁,叶绿体,二次代谢产物和核酶.
  • 流细胞计为植物细胞核或染色体的高效净化提供了一个解决方案.

研究的目的:

  • 提出详细的方案,用于从植物中准备HHMWDNA,使用流细胞计.
  • 为了使各种植物物种的全基因组和染色体光学图的构建.

主要方法:

  • 利用流式细胞计量来快速有效地净化植物细胞核或基因相染色体.
  • 将净化核/染色体嵌入阿加罗斯插头中,以在现场分离HMW DNA.
  • 应用协议来生成光学地图.

主要成果:

  • 成功开发了一种流量分类辅助的协议,用于植物中的HMW DNA制备.
  • 证明了该协议在构建全基因组和染色体光学图的有效性.
  • 在多个家族的20种植物物种中验证了该方法.

结论:

  • 流细胞计是克服HMWDNA隔离中植物特异性挑战的关键启用技术.
  • 该协议可在广泛的植物物种中进行高分辨率光学映射.
  • 该方法支持先进的植物基因组研究,包括组装和结构变异研究.