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在烟草中通过CRISPR/Cas9介导的线粒体基因组编辑诱导男性不育

Yanzi Chang^1,2,3, Baolong Liu^1,2,3, Yanyan Jiang^1,2,4

¹Qinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, Qinghai, China.

Functional & integrative genomics

|June 19, 2023

概括

使用CRISPR/Cas9技术修改了植物线粒体基因组,通过向mtatp9基因成功创建了一个男性无菌烟草突变体. 这一突破表明了线粒体基因组编辑在植物中的潜力.

科学领域:

植物分子生物学植物分子生物学
线粒体遗传学线粒体遗传学
基因组编辑基因组编辑

背景情况:

CRISPR/Cas9技术在各种系统中彻底改变了基因组编辑.
植物线粒体基因组修改仍然具有挑战性.
植物中的细胞质男性不育性 (CMS) 与线粒体基因有关,但直接基因修饰尚未得到充分研究.

研究的目的:

研究利用CRISPR/Cas9.9针对植物线粒体基因组的可行性.
确定mtatp9基因在烟草中细胞质男性不育 (CMS) 的作用.
在植物中建立直接线粒体基因修饰的方法.

主要方法:

导向线粒体定位信号的CRISPR/Cas9 (mitoCRISPR/Cas9) 被用来准烟草中的mtatp9基因.
分析了基因编辑效率和表型效应在导致的突变.
进行了转录组分析,以了解男性无菌突变体的代谢变化.

主要成果:

在烟草的线粒体基因组中,mtatp9基因被成功切割,导致堕胎的干细胞导致男性不育.
男性无菌突变体显示了mtDNA拷贝数量的减少和异质体mtatp9等位基因百分比的改变,种子设置率为零.
代谢途径包括糖解,三碳酸循环和氧化酸化在突变菌株中被抑制.
过度表达dsmtatp9恢复了生育能力,证实了mtatp9在CMS中的作用.

结论:

mtatp9基因的突变是烟草中细胞质男性不育 (CMS) 的原因.
mitoCRISPR/Cas9是针对植物线粒体基因组的有针对性的修改的一个有效工具.
这项研究为了解和操纵植物中的线粒体功能提供了基础.

关键词:

男人的不孕不育能力.这就是MitoCRISPR/Cas9.MtDNA编辑编辑 MtDNA编辑烟草烟草烟草烟草烟草 mtatp9 在线观看

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