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迄今为止最大的类动物参考基因组组合:西部,Sceloporus occidentalis

Anusha P Bishop^1,2, Erin P Westeen^1,2, Michael L Yuan³

¹Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, CA, United States.

The Journal of heredity

|June 19, 2023

概括

使用先进的测序技术组装了西部 (Sceloporus occidentalis) 的新参考基因组. 这种基因组将有助于理解的进化和保护,特别是对于岛屿种群.

科学领域:

基因组学就是基因组学.
生态生态学生态生态学
进化生物学进化生物学

背景情况:

刺 (Sceloporus属) 是生态和进化研究的关键模型.
西方 (Sceloporus occidentalis) 面临着气候和土地利用变化的威胁.
需要一个高质量的参考基因组是Sceloporus occidentalis.

研究的目的:

为了生成一个高质量的新基因组组装Sceloporus occidentalis.
为了支持加利福尼亚保护基因组学项目 (CCGP) 内的保护基因组学努力.
为研究Sceloporus的生态和进化动态提供资源.

主要方法:

利用太平洋生物科学HiFi长读取基因组测序.
使用Hi-C染色体近距离测序来进行基因组组装.
进行了Sceloporus occidentalis基因组的新组装.

主要成果:

产生了一个由608个支架组成的基因组组合,总计2856 Mb.
实现了18.9 Mb的contig N50和98.4 Mb的脚手架N50.
在四足动物基因组中获得了98.1%的BUSCO完整度得分.

结论:

这种新的参考基因组是Sceloporus occidentalis研究的宝贵资源.
促进对物种状况的研究,例如岛 (S. becki).
能够更深入地了解Sceloporus属内的进化辐射.

关键词:

这是CCGP的CCGP.加利福尼亚州保护基因组学项目伊瓜尼亚伊瓜尼亚伊瓜尼亚这就是Phrynosomatidae.斯克瓦马塔 (Squamata) 是一个小队.在 de novo 基因组组装中.爬行动物爬行动物爬行动物

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