在健康个体和易怒肠综合征患者的血液中进行甲基转录组分析
Lauma Jagare1, Maija Rozenberga1, Ivars Silamikelis1
1Latvian Biomedical Research and Study Centre, Human Genetics and Disease Mechanisms Group, Ratsupites iela 1, Riga, LV-1067, Latvia.
Journal of medical microbiology
|June 19, 2023
概括
血液微生物组,以前没有活跃的微生物转录的特征,在患有易怒肠综合征 (IBS) 的患者中显示出明显的差异. 这项研究表明,肠道微生物组会影响血液微生物组,特别是在肠道透性增加的情况下.
科学领域:
- 微生物学 微生物学
- 人类微生物组研究研究
- 胃肠病学 胃肠病学
背景情况:
- 人类血液微生物组的概念正在出现,越来越多的证据表明其存在.
- 之前的研究主要使用DNA测序,不研究活跃的微生物成分及其与肠道透性的联系.
研究的目的:
- 在血液中检测活跃的微生物使用metatranscriptomics.
- 为了研究健康个体和患有刺激性肠综合征 (IBS) 的患者之间血液微生物群的分类学差异.
主要方法:
- 从23名IBS患者和26名健康对照人群中采集了血液样本.
- 进行RNA测序 (RNAseq) 来分析微生物转录.
- 使用Kraken 2和Bracken 2进行了分类学识别,比较IBS和对照组.
主要成果:
- 在血液微生物组中确定的主要属包括Cutibacterium,Bradyrhizobium和Escherichia.
- 与肠道相关的属 (例如,Mediterraneibacter,Blautia,Faecalibacterium) 不太可能受到污染,并且在IBS患者中更为普遍.
- 特定的肠道微生物群 (Blautia,Faecalibacterium,Dorea,Bifidobacterium,Clostridium,Christensenella) 在IBS患者中显示出更高的患病率.
结论:
- 这些发现支持活跃的血液微生物组的存在.
- 肠道微生物组被认为是主要来源,口腔和皮肤微生物组作为潜在的次要来源.
- 血液微生物组似乎受到肠道透性增加的条件的影响,例如IBS.
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