在玉米内发育期间的自会减轻氧化应激,并促进线粒体清除
Jessica A S Barros1, Elizabeth C Chatt1, Robert C Augustine1
1Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.
Plant physiology
|June 19, 2023
概括
自对玉米种子的营养储存不至关重要,但可以防止氧化应激. 宏观自突变体表现出改变的代谢和功能障碍的线粒体,这表明它们在种子发育过程中在细胞质量控制中的作用.
科学领域:
- 植物生物学 植物生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 自是一种细胞过程,用于回收组件并维持恒常状态.
- 了解自在种子发育中的作用对于作物改善至关重要.
研究的目的:
- 调查宏自对玉米 (Zea mays) 内的成熟和营养储存的影响.
- 分析自性缺陷玉米内的代谢,转录和蛋白质变化.
主要方法:
- 使用了一种集成的多原子方法 (转录组学,蛋白质组学,代谢组学).
- 研究了缺乏核心宏自因子ATG12的玉米内突变.
- 检查了种子成熟的早期和中期发育阶段.
主要成果:
- 自缺陷的玉米内积累了正常的粉和Zain蛋白质.
- 代谢组的显著变化,特别是氧化应激和硫代谢途径的变化.
- 线粒体蛋白质增加而没有相应的mRNA变化,以及功能障碍的线粒体和减弱的线粒体衰变的证据.
结论:
- 麦克罗自在粉和储存蛋白质在玉米内的积累中起到较小的作用.
- 自对于防止氧化应激和清除玉米种子发育过程中功能失调的线粒体而言很重要.
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