使用MitoQQ保护解后精子的质量和生育潜力
P Alipour-Jenaghard1, H Daghigh-Kia1, R Masoudi2
1Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Theriogenology
|June 19, 2023
概括
用抗氧化剂MitoQ补充精液冷保存,可显著改善解后的精子质量和生育能力. MitoQ保留了运动性,膜完整性和活力,同时减少了冷解的精子中的氧化应激和DNA损伤.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
背景情况:
- 精子的冷保存对于商业家禽养殖至关重要.
- 冷解过程对精子质量和生育能力产生负面影响.
- 需要有效的方法来缓解精子的冷损伤.
研究的目的:
- 评估针对线粒体的抗氧化剂MitoQ在冷保存后保护精子质量和生育能力方面的有效性.
- 确定MitoQ的最佳度,以在冷解过程中保护精子.
主要方法:
- 的精液被冷保存在湖水中,并补充了不同度的MitoQ (0,1,10,100,1000nm).
- 评估了解后的精子参数,包括运动性,膜功能,形态,线粒体潜力,体完整性,活力,亡,脂质过氧化,DNA碎片化,ROS和生育能力.
- 进行了统计分析,以比较不同MitoQ度的影响.
主要成果:
- 与对照组相比,用10和100nM的MitoQ补充剂显著改善了总运动性,渐进运动性,精子速度,膜功能,线粒体潜力,体完整性和活力.
- 在10和100 nM MitoQ组中观察到较低的脂质过氧化,亡,DNA碎片化和活性氧物种 (ROS).
- 在接受10和100nM MitoQ.治疗的组中,生育率显著更高.
结论:
- MitoQ有效地保持了冷保存的精子的质量和生育潜力.
- 在10和100nM的度下,MitoQ是精液冷保存介质的有益添加剂.
- 这一发现支持在家禽繁殖计划中使用MitoQ,以提高精液活力和生育结果.
相关概念视频
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:15Sperm Transport
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
01:22Sperm Structure and Semen Composition
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
01:17Male Sexual Response: Erection & Ejaculation
Sexual stimulation can take various forms, such as physical touch and visual or auditory cues. When this happens, the parasympathetic reflex in the sacral portion of the spinal cord is activated. This reflex stimulates the release of nitric oxide (NO), which then dilates the arterioles in the penis, increasing blood flow to the erectile tissues - the corpora cavernosa and corpus spongiosum.
The blood filling the erectile tissues compresses the veins, which helps to prevent blood from leaving...
The blood filling the erectile tissues compresses the veins, which helps to prevent blood from leaving...
01:23Infertility in Males
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...