免疫分析和RNA-seq揭示了部分不明原因的复发性植入失败的原因
Xiangxiu Fan1, Qi Zhao1, Yuan Li2
1An Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University Changsha, China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
International immunopharmacology
|June 19, 2023
概括
子宫内膜中的免疫细胞过度激活与复发性植入失败 (RIF) 有关. 这项研究确定了RIF患者的免疫细胞比例升高,有助于患者的分类和理解RIF机制.
科学领域:
- 生殖免疫学 生殖免疫学
- 子宫内膜生物学子宫内膜生物学
- 基因组学就是基因组学.
背景情况:
- 对于无法解释的复发性植入失败 (RIF) 中子宫内膜免疫细胞动态的有限理解.
- 在植入窗口期间,需要阐明RIF患者的免疫细胞功能和相互作用.
研究的目的:
- 将患者分类为不明原因的RIF.
- 通过子宫内膜免疫分析和RNA测序来研究RIF背后的机制.
主要方法:
- 注册了172名参与者:有144名没有解释的RIF和28名肥沃的对照.
- 收集的子宫内膜样本在中期的流产阶段.
- 对关键免疫细胞 (NK,巨细胞,T,B细胞) 进行了转录组测序和免疫组织化学染色.
主要成果:
- 在RIF患者中,子宫NK细胞 (CD56+),NKT细胞 (CD57+),巨细胞 (CD68+) 和B细胞 (CD19+) 的比例显著增加.
- 与对照人群相比,RIF患者在腺体光线内增加了CD68+巨细胞.
- 根据发现,为RIF患者开发了一个三类分类系统.
结论:
- 子宫内膜免疫细胞过活化可能会降低子宫内膜耐受性.
- 这种免疫失调可能与反复的植入失败和不良妊娠结果有关.
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