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在干旱压力转录组中挖掘的小开放阅读框架 (sORFs) Oryza sativa亚种. 标志性标志性标志性标志性

Sheue Ni Ong¹, Boon Chin Tan¹, Kousuke Hanada²

¹Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.

|June 19, 2023

概括

这项研究研究了干旱压力下的大米中小型开放式读取框架 (sORF). 研究人员确定了参与防御和新陈代谢的差异表达的sORF,突出了对气候适应性大米发展的潜力.

科学领域:

植物科学植物科学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

干旱是影响全球大米生产的主要非生物压力.
虽然研究了干旱下的大米转录组学,但小开放阅读框架 (sORF) 调节仍然未得到充分探索.
不同的干旱强度会在植物中触发不同的调节机制.

研究的目的:

在不同的土壤含水量 (SMC) 条件下,研究大米中sORFs的差异表达.
为了确定参与干旱应激反应途径的sORF.
探索已识别的sORF的潜力,以开发适应气候变化的米品种.

主要方法:

在四叶阶段,大米幼苗遭受了两个干旱压力水平:40%和30%的土壤水分含量 (SMC).
进行RNA测序以分析干旱条件下的sORF表达变化.
使用NanoString技术验证了差异表达.

主要成果:

总共有122个和143个差异表达的sORF分别在40%和30%的SMC下被确定.
基因本体学 (GO) 丰富分析显示,sORF参与了防御反应和细胞平衡.
基因和基因组的京都百科全书 (KEGG) 途径分析将DE sORF与托和氨酸代谢联系起来.

结论:

干旱压力显著改变了大米中的sORF表达特征.
已识别的DE sORF在与应激反应和新陈代谢相关的关键生物过程中发挥作用.
响应干旱压力的非特征性sORFs代表了提高大米气候弹性的有希望的目标.

关键词:

干旱造成的压力是干旱.印度菜大米 (Indica Rice) 是一种印度菜大米.在RNA-Seqq.小的开放式阅读框架.转录组转录组就是一个转录组.

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