在使用细胞技术修复切除的肝脏后,可能出现脏并发症
I V Maiborodin1,2, R V Maslov3, S V Marchukov3
1Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia. imai@mail.ru.
Bulletin of experimental biology and medicine
|June 19, 2023
概括
注射到老鼠肝脏的骨髓 stromal 细胞 (MSC) 导致损伤,包括管状变化和感染. 在脏中没有发现MSC或其副产品,这表明间接影响. 建议在肝细胞治疗中仔细考虑脏血栓形成风险.
科学领域:
- 再生医学是一种再生医学.
- 细胞疗法细胞疗法
- 脏病理学 脏病理学
背景情况:
- 骨髓多能性树皮细胞 (MSCs) 正在探索治疗潜力.
- 了解MSC的全身影响,特别是对远离注射部位的器官的影响,至关重要.
研究的目的:
- 为了研究注射到肝脏的老鼠骨髓多能干细胞 (MSCs) 对脏结构和功能的影响.
- 为了确定MSC或其降解产品在肝服后是否迁移到脏.
主要方法:
- 在注射Vybrant CM-Dil标记的MSCs到完整或切割的肝脏后,使用光显微镜检查了老鼠脏.
- 在注射后的不同时间点评估脏组织的组织病理变化.
主要成果:
- 脏变化包括颗粒性缩和细胞死亡在远端管道和收集管道,以及血管血栓形成和上升性尿路感染.
- 在任何观察点都没有在脏中检测到MSC或其降解产物.
- 后来在MSC注射到部分切除的肝脏后,观察到髓中的细菌污染.
结论:
- 肝脏给药MSCs可以导致间接脏损伤,可能由免疫调节作用介导.
- 血管血栓是肝脏MSC注射的潜在并发症,需要在细胞治疗方案中考虑.
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