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大豆免疫受体GmBIR1在囊线虫感染期间调节宿主转录组,结合组和免疫力

Tracy E Hawk¹, Sarbottam Piya¹, Sobhan Bahrami Zadegan^1,2

¹Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA.

The New phytologist

|June 20, 2023

概括

大豆BAK1交互受体LikeKINASE1 (BIR1) 负面调节植物免疫力. GmBIR1通过改变基因表达和替代拼接来增强对大豆囊线虫 (SCN) 的敏感性.

科学领域:

植物分子生物学植物分子生物学
植物与微生物的相互作用
植物免疫力植物免疫力

背景情况:

像kinase1 (BIR1) 这样的BAK1交互受体是已知的植物免疫反应的负调节者.
了解BIR1在大豆 (Glycine max) 免疫力中对诸如大豆囊线虫 (SCN) 等病原体的作用,对于作物保护至关重要.

研究的目的:

研究大豆GmBIR1在大豆-SCN相互作用中的功能作用.
阐明GmBIR1调节植物免疫力的分子机制,重点关注转录组和结合组的改变.

主要方法:

在转基因大豆毛根中过度表达野生类型 (WT-GmBIR1) 和激酶死 (KD-GmBIR1) 变体.
转录组分析 (RNA-seq) 来识别差异表达的基因.
定量蛋白组学用于识别GmBIR1信号通路基质.
拼接事件的全基因组分析.

主要成果:

过度表达WT-GmBIR1增加了SCN易感性,而KD-GmBIR1增加了耐药性.
转录组分析揭示了防御和免疫基因的丰富,这些基因由WT-和KD-GmBIR1.1.2相反调节.
蛋白组分析确定了许多假定的GmBIR1基质,其中许多在SCN感染时显示差异酸化.
证据支持GmBIR1在SCN感染期间调节替代性mRNA前拼接中的作用.

结论:

GmBIR1作为大豆对SCN免疫力的负调节剂.
GmBIR1信号通路通过对拼接因子的差异酸化影响大豆转录组和拼接组.
GmBIR1调节了前mRNA衰变和结合体相关基因的替代拼接,为植物免疫调节提供了新的见解.

关键词:

一个 BIR1 一个 BIR1 替代性拼接是一种替代性的拼接.免疫反应的免疫反应.素蛋白质组学素蛋白质组学素蛋白质组学豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆大豆囊线虫豆囊线虫转录组 (transcriptome) 是一个转录组.

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