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通过共价和非共价修饰制造的肌纤维蛋白-丁合物之间的比较

Zhenyang Wu¹, Xue Zhao¹, Xinglian Xu¹

¹State Key Laboratory of Meat Quality Control and Cultured Meat Development; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, PR China.

Journal of agricultural and food chemistry

|June 20, 2023

概括

肌纤维蛋白 (MP) - - 卢托林 (Lut) 结合物通过非共价键和共价键形成. 结合模式没有显著改变抗氧化剂活性,为功能性食品应用提供了洞察力.

科学领域:

食品科学食品科学食品科学
生物化学生物化学
蛋白质化学蛋白质化学

背景情况:

蛋白质 - 黄类化合物的结合可以增强蛋白质的功能.
了解结合模式对于优化并联性质至关重要.
非共价与共价结合对蛋白质-黄类结合物的影响尚不清楚.

研究的目的:

调查非共价和共价结合方式对肌纤维蛋白 (MP) - 卢托林 (Lut) 结合体构成和抗氧化特性的影响.
阐明MP-Lut结合体形成中涉及的结合力和位点.
为应用MP-Lut复合物作为功能成分提供理论基础.

主要方法:

使用不同Lut度的MP与Lut的非共价和共价结合.
光火用于确定结合力和热力学.
液体染色学 - 双重质谱学 (LC-MS/MS) 用于对应结合的确认.
蛋白质组学分析以确定蛋白质移植部位.
在体外抗氧化剂活性测定.

主要成果:

非共价MP-Lut结合主要由疏水性相互作用主导,并且由驱动.
在性处理后证实了Lut对MP的共接种,主要是在肌肉素子单元上.
MP-Lut结合物的抗氧化活性在很大程度上不受结合方式 (非共价与共价) 的影响.

结论:

非共价和共价结合策略都可以成功地形成MP-Lut联物.
结合模式不会显著影响结合物的抗氧化能力.
这项研究为在功能应用中利用MP-Lut非共价/共价复合体提供了理论基础.

关键词:

抗氧化剂活性抗氧化剂活性同价值变化的修改.黄类蛋白质结合物是黄类蛋白质的结合物.非对应性修改的非对应性修改

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