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来自热性菜的糖载体SrSTP14的分子特征及其在培养花粉中的可能作用

Daiki Koyamatsu¹, Miyabi Otsubo¹, Tomonori Ohira¹

¹Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.

Physiologia plantarum

|June 20, 2023

概括

亚洲菜利用糖载体SrSTP1和SrSTP14进行花热生成. 这些载体促进了黑色素的吸收,SrsTP14可能有助于花粉的发育.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

植物热生成对于植物繁殖至关重要,它涉及到用于能量和发育的糖.
热生植物中糖运输的机制尚未完全理解.
亚洲菜 (Symplocarpus renifolius) 呈现出强烈的花热量产生.

研究的目的:

研究糖载体 (STPs) 在亚洲菜的热生成中的作用.
描述两个特定的STPs,SrSTP1和SrSTP14的功能和定位.

主要方法:

使用RNA-seq和实时PCR进行基因表达分析.
功能补充测定在缺乏赫索斯载体的酵母菌株中.
使用原生质和GFP融合蛋白中短暂表达的亚细胞局部化研究.
通过in situ杂交进行组织特异性表达分析.

主要成果:

SrSTP1和SRSTP14基因在骨的热生成过程中被上调,主要是在骨中.
无论是SrSTP1还是SrSTP14,都促进了酵母酵母中体 (葡萄糖和银河糖) 的吸收.
SrSTP1 和 SrSTP14 蛋白位于菜原生体中的血膜上.
在发育的微胞子中检测到SrSTP14mRNA,在发热阶段.

结论:

SrSTP1和SRSTP14是血局部化的体转运体,参与亚洲菜热生成.
通过调解前体细胞中色体的吸收,SrSTP14可能对花粉发育至关重要.

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相关概念视频

Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes: