一种MYB激活剂,DcMYB11c,可以调节胡卜的安东基氨酸在树枝中的积累,但不能调节树根的积累
Ao-Qi Duan1, Yuan-Jie Deng1, Shan-Shan Tan1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Plant, cell & environment
|June 20, 2023
概括
DcMYB11c 是一个关键基因,它调节紫色胡卜树叶中的安东素颜料. 它的过度表达会导致紫色变色,而它的淘汰会减少它,揭示了它在酸氨酸生物合成中的关键作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 负责紫色染色的颜料安托西亚宁在植物发育和健康方面至关重要.
- 早期的化胡卜很可能是紫色的,这表明对抗生素生产的遗传基础.
- 胡卜中的P3区域含有MYB基因的集群,这些基因参与了氨酸生物合成.
研究的目的:
- 识别和描述DcMYB11c在胡卜树枝中安东素颜色的作用.
- 阐明DcMYB11c在氨酸生物合成中的调节机制.
- 为了研究胡卜树枝中紫色染色的遗传基础.
主要方法:
- 在不同胡卜组织中对DcMYB11c的基因表达分析.
- 在胡卜品种中,DcMYB11c的过度表达和CRISPR/Cas9介导的淘汰.
- 酵母单杂交和双化酶记者测试以确定基因相互作用和促进体结合.
主要成果:
- DcMYB11c在紫色胡卜树叶中高度表达,其过度表达导致了安托西亚尼的积累和紫色的颜色.
- 淘汰DcMYB11c显著降低了安东素含量,并导致浅紫色的表型.
- DcMYB11c直接激活了包括DcUCGXT1和DcSAT1以及DcbHLH3.3.在内的氨酸生物合成基因的表达.
结论:
- DcMYB11c 是一个关键的转录因子,调节胡卜树中的安托西亚宁生物合成.
- 这项研究揭示了胡卜中安东素积累的精确调节机制.
- 这些发现提供了关于植物物种中保存的安托素调节途径的见解.
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