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μPESI-MS/MS 系统用于查和定量血样本中的药物

Simin Zhang¹, Qian Liu^1,2, Xiangyang Qu¹

¹Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong China, 510006.

Journal of the American Society for Mass Spectrometry

|June 20, 2023

概括

一种新的微探针电喷离离子与联质谱学 (μPESI-MS/MS) 系统相结合的新型微探针电喷离子被验证用于血中药物定量. 这种方法表现出强的性能,特别是在具有较高脂性和电荷的药物中.

科学领域:

分析化学分析化学
质谱测量质量谱测量
药理学药理学是指药理学的学科.

背景情况:

在生物矩阵中对药物的定量分析对于治疗药物监测和药理动力学研究至关重要.
现有的方法可能需要大量的样本准备或专门的设备.
此前已经开发了一种新型的微探针喷射电离电离 (μPESI) 源,并配备了双重质谱 (MS/MS).

研究的目的:

验证μPESI-MS/MS方法用于血样本中药物的定量分析.
研究药物物理化学特性与μPESI-MS/MS方法的定量性能之间的关系.

主要方法:

为5种代表性药物开发和验证μPESI-MS/MS方法.
对线性,准确性和精度的评估与欧洲药品管理局 (EMA) 的指导方针相比.
在血样本中分析了75种药物,重点是定量性能和物理化学性质 (log P,pKa,电荷).

主要成果:

μPESI-MS/MS方法满足了EMA对测试药物的线性,准确性和精度的要求.
在75种药物中,48种药物在血样本中成功量化.
后勤回归表明,较高的log P值和生理负荷与更好的定量性能正相关.

结论:

PESI-MS/MS系统是一种经过验证和实用的方法,用于在血中快速定量药物.
药物的物理化学性质,特别是脂性和电荷,影响μPESI-MS/MS方法的定量性能.
这种技术为临床和研究环境中高通量药物分析提供了有前途的方法.

关键词:

生物分析是一种生物分析.微探针电喷离子化合式质谱学质谱学探测器电喷射电离化的电离.

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