从临床和分子机制确定NUDCD1在人类瘤中的作用:基于综合生物信息学和实验验证的研究
Bin Han1,2,3, Jinsong He4, Qing Chen1,3,4
1GCP Center/Institute of Drug Clinical Trials, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Aging
|June 20, 2023
概括
含NudC域1 (NUDCD1) 在大多数癌症中表达很高,并且与预后不佳有关. 这项研究提供了NUDCD1的第一个泛癌症分析,揭示了它在免疫反应,药物敏感性和癌症途径,特别是胃腺癌 (STAD) 中的作用.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 含NudC域1 (NUDCD1) 是一种已识别的癌症抗原,在多种瘤中具有异常激活.
- 目前还没有对NUDCD1在人类癌症中的作用进行全面的泛癌症分析.
研究的目的:
- 对NUDCD1表达,基因变异和人类癌症的临床意义进行第一个泛癌症分析.
- 研究NUDCD1与免疫透,药物敏感性和癌症相关途径的关联.
- 在胃腺癌 (STAD) 中实验验证NUDCD1的功能.
主要方法:
- 使用公共数据库 (HPA,TCGA,GEO,GTEx,TIMER2,TISIDB,UALCAN,GEPIA2,cBioPortal,GSCA) 进行胰腺癌分析.
- 进行了分子实验,包括定量实时PCR,免疫组织化学和用于STAD验证的西部斑.
- 分析了NUDCD1表达,遗传/表观遗传特征,免疫细胞透,药物敏感性和通路丰富.
主要成果:
- 在大多数瘤中,NUDCD1的表达很高,并且与更差的预后有关.
- 在NUDCD1和免疫检查点 (例如,抗CTLA-4) 和免疫透物 (CD4 +,CD8 + T细胞) 之间发现了显著的相关性.
- NUDCD1证明了与药物敏感性的联系,并参与了关键的癌症途径,如细胞亡,细胞循环和DNA损伤,在STAD.实验中进行了实验验证.
结论:
- NUDCD1在各种癌症中发挥着重要作用,影响瘤发育,免疫逃避和治疗反应.
- 这种泛癌分析确定了NUDCD1作为潜在的泛癌生物标志物和治疗点,特别是在STAD.
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