相关实验视频
Updated: Jul 26, 2025

08:46
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
Published on: August 12, 2020
6.5K
B细胞激活因子有助于提高新生儿败血症中调控性B细胞的含量
Bulletin of experimental biology and medicine
|June 20, 2023
概括
在新生儿败血症中增加的B细胞激活因子 (BAFF) 通过PI3K/AKT/mTOR途径促进调控性B细胞 (Breg) 分化. 这一发现突出了BAFF的发现.
科学领域:
- 免疫学 免疫学 免疫学
- 新生儿研究新生儿研究
- 分子生物学分子生物学
背景情况:
- 调控性B淋巴细胞 (Breg) 在免疫平衡中起着至关重要的作用.
- 新生儿的败血症会破坏免疫调节,导致不良结果.
- B细胞激活因子 (BAFF) 和PI3K/AKT/mTOR通路与B细胞功能有关.
研究的目的:
- 研究BAFF在PI3K/AKT/mTOR信号通路中的作用.
- 确定BAFF如何影响毒性新生儿中调控性B淋巴细胞 (Breg) 的增殖和存活.
- 检查新生儿败血症中BAFF水平及其受体表达.
主要方法:
- 从40名患有败血症的早产新生儿和40名匹配的对照人群中采集了外周血液样本.
- 用LPS和CpG-oligodeoxynucleotide (CpG-ODN) 来分离和刺激B细胞.
- 使用流式细胞计,qRT-PCR和西部抹杀分析B细胞分化,Breg群体 (CD19+CD24hiCD38hi) 和PI3K/AKT/mTOR通路激活.
主要成果:
- 与对照组相比,败血症新生儿的BAFF水平显著增加,BAFF受体表达的平行增加.
- 在实验室中,BAFF刺激促进了B细胞分化为CD19+CD24hiCD38hi Breg细胞.
- 在BAFF刺激时观察到PI3K/AKT/mTOR通路的激活,4E-BP1和70S6K的酸化增加表明了这一点.
结论:
- 新生儿败血症中BAFF水平升高会激活PI3K/AKT/mTOR信号通路.
- 这种激活导致外周血液B细胞在体外分化成CD19+CD24hiCD38hi Breg细胞.
- 在新生儿败血症的背景下,BAFF在布雷格细胞的发育和生存中发挥着重要作用.
相关概念视频
B Cell Activation and Differentiation
1.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.8K
Development of Immunocompetence
366
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
366
Defense Against Bacterial Pathogens
1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K
Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
NF-κB-dependent Signaling Pathway
7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.5K
Humoral Immune Responses
73.3K
Overview
73.3K

