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重编程人类女性脂肪性介质细胞干细胞变成原始生殖细胞类细胞

Giulia Salvatore¹, Susanna Dolci¹, Antonella Camaioni¹

¹Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, Rome, 00133, Italy.

Stem cell reviews and reports

|June 20, 2023

概括

雌性人类脂肪衍生性树皮细胞 (hASCs) 可以在特定的预诱导后产生原始生殖细胞样细胞 (PGCLCs). 然而,使用来自hASCs的诱导多能干细胞对于PGCLC生成更有效.

科学领域:

干细胞生物学干细胞生物学
生殖生物学生殖生物学
细胞分化的细胞分化.

背景情况:

在从像ESC和iPSC这样的多能干细胞中获得哺乳动物生殖细胞方面取得了重大进展.
多能干细胞通常在分化为PGC类细胞 (PGCLCs) 之前被引导到前胃化内皮/半皮状状态.
脂肪酸衍生性树皮细胞 (ASC) 是能够分化为各种细胞类型的多能细胞,但它们的生殖细胞衍生潜力尚未被探索.

研究的目的:

研究女性人类ASCs (hASCs) 产生PGCLCs的能力.
为了比较直接从hASCs与hASC衍生的iPSCs生产PGCLC的协议.

主要方法:

人类ASCs接受了诱导前的协议,以促进周胃流动的内皮/半皮状状态.
我们比较了从hASC直接生成PGCLC的协议.
他们比较了从hASC生成PGCLC的协议,这些hASC首先被重新编程成诱导多能干细胞 (iPSC).

主要成果:

人类ASCs可以产生PGCLCs当预诱导到一个围胃化内皮/半皮类似的状态.
与使用hASC衍生的iPSC相比,直接从hASC生成PGCLC的效率较低.
尽管表达了中皮基因并具有多能性,但从hASCs直接诱导PGCLCs的效率较低.

结论:

人类雌性ASC在适当的预诱导后具有将其分化为PGCLC的潜力.
从hASC衍生出的iPSC作为产生PGCLC的更有效的起始材料,而不是从hASC直接分化.
对于直接hASC-to-PGCLC分化协议,可能需要进一步优化.

关键词:

脂肪酸衍生中酶体干细胞的干细胞.人工性交配体的人工性交配体人类不孕症人类不孕症在PGCLCs中,PGCLCs是最重要的.原始的生殖细胞.

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