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Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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Updated: Jun 16, 2026

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Published on: December 9, 2022

优化线性离子陷数据独立获取,以实现单细胞蛋白质组学.

Teeradon Phlairaharn^1,2,3, Zilu Ye¹, Elena Krismer¹

¹The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, København 2200, Denmark.

Analytical chemistry

|June 20, 2023

概括

线性离子陷 (LIT) 现在可以执行全面的蛋白质组学,包括图书馆生成,而不需要依赖其他质量分析仪. 这一进步使得低输入样本和单细胞分析能够准确量化到0.5nG.

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Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

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Published on: April 4, 2018

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科学领域:

质谱测量质量谱测量
蛋白质组学是指蛋白质组学.
分析化学分析化学

背景情况:

线性离子陷 (LIT) 提供了可负担性,稳定性和速度,但与飞行时间或轨道陷分析仪相比,其质量准确性有限.
以前使用LIT的低输入蛋白质组学研究通常需要从轨道飞行仪器或基于轨道飞行图书馆获得补充数据.
本研究探讨了LIT在低输入蛋白质组学中的所有质谱测量的独立能力.

研究的目的:

证明LIT作为低输入蛋白质组学独立质量分析仪的多功能性.
优化LIT数据采集和图书馆生成方法,以进行敏感和准确的蛋白质组分析.
评估基于LIT的定量蛋白质组学的性能,包括定量限度和单细胞应用.

主要方法:

为蛋白质组学优化LIT数据采集参数.
无图书馆数据库搜索带有和没有捕获的数据库,以评估检测和量化.
生成矩阵匹配的校准曲线来确定量化下限 (LLOQ).
开发用于低输入样本的光谱库生成策略.
应用LIT-DIA (数据独立获取) 用于使用LIT生成的库进行单细胞蛋白质组学分析.

主要成果:

在LIT-MS1测量中,定量准确度较差,而在LIT-MS2测量中,定量准确度降至0.5 ng.
仅使用 10 ng 的起始材料估计了下限量化值.
一个强大的光谱库生成策略被优化为低输入材料.
使用LIT-DIA成功分析了单细胞样本,图书馆来自40个细胞.

结论:

线性离子陷可以作为多功能,独立的质量分析仪,用于全面的低输入蛋白质组学,包括图书馆生成.
LIT-MS2测量提供了精确的量化,对于分析微小的生物样本至关重要.
这种方法使单个细胞的敏感蛋白质基因分析成为可能,扩大了负担得起的质谱平台的实用性.