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聚化控制了肺炎球菌性肺炎小鼠模型中髓状细胞的免疫功能

Prajakta Shinde¹, Alexander Kiepas², Lei Zhang¹

¹Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

|June 20, 2023

概括

缺少聚酸 (polySia) 的小鼠对斯特雷普菌肺炎感染的敏感性降低. 这些缺乏polySia的小鼠更快地清除细菌,改善了巨细胞功能,但改变了白细胞迁移.

科学领域:

免疫学免疫学免疫学
葡萄糖生物学葡萄糖生物学
细胞生物学细胞生物学

背景情况:

聚酸 (polySia) 是细胞表面蛋白质的翻译后修饰,影响细胞相互作用.
在细菌感染期间,polySia在白细胞功能中的作用仍然在很大程度上未被描述.

研究的目的:

为了研究polySia缺乏对宿主免疫反应对Streptococcus pneumoniae (Spn) 感染的影响.
阐明在SPN感染期间白细胞行为和功能中polySia的作用.

主要方法:

评估多Sia缺乏ST8SiaIV^-/-小鼠和野生类型 (WT) littermates感染了肺炎杆菌.
评估细菌清除,白细胞肺部招募,膜巨细胞活动和细胞信号通路 (ERK1/2).
利用采用细胞转移,微流体迁移试验和静脉内显微镜来研究白细胞迁移.

主要成果:

与WT小鼠相比,ST8SiaIV^-/-小鼠对SPN感染的敏感性降低,细菌清除速度更快.
在ST8SiaIV^-/-小鼠中,膜巨体显示出增强的生存能力和细胞活性.
矛盾的是,白细胞肺部招募在感染ST8SiaIV^-/-小鼠中减少,这与失调的ERK1/2信号相关.

结论:

聚酸在细菌感染期间调节白细胞反应方面发挥着多方面的作用.
丢失的polySia增强了巨细胞的功能,但损害了白细胞的迁移,这表明复杂的免疫调节效应.
研究结果表明,polySia是优化感染期间免疫反应的潜在治疗点.

关键词:

CP: 免疫学免疫学肺炎链球菌的肺炎链球菌.细胞外信号调节的激酶ERK1/2 静脉内显微镜检查静脉内显微镜检查白血球迁移的流动性微流体迁移室中的微流体单细胞 (Monocytes) 是一种单细胞.中性粒细胞中性粒细胞.发酵细胞的形成发酵细胞的形成聚氨酸酸是什么? 聚氨酸是什么聚甲基转移酶ST8SiaIV 聚甲基转移酶

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