从泰国使用Clostridium beijerinckii基于ATCC 10132的ABE发酵的海洋大藻生产布他诺的潜力
Sireethorn Khaonuan1,2, Rattana Jariyaboon2,3, Nikannapas Usmanbaha1,2
1Energy Technology Program, Faculty of Engineering, Prince of Songkla University, Songkhla, Thailand.
Biotechnology journal
|June 20, 2023
概括
海洋大藻为生物butanol生产提供了一个可持续的来源. Gracilaria tenuistipitata对乙-布坦醇-乙醇发酵具有前景,优化的预处理产生了显著的结果.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 海洋生物学 海洋生物学
背景情况:
- 第一代和第二代生物质对生物butanol生产具有成本限制.
- 第三代生物质,特别是海洋大藻,为可持续生物燃料生产提供了潜在的替代方案.
- 乙-butanol-乙醇 (ABE) 发酵是将生物质转化为生物butanol的一个关键过程.
研究的目的:
- 通过使用Clostridium beijerinckii.比较评估三种海洋大藻类的butanol产量.
- 优化 Gracilaria tenuistipitata 的低温水热预处理 (HTP),以提高生物butanol 的产量.
- 为了评估在ABE发酵中预处理的大藻的效率.
主要方法:
- 从Gracilaria tenuistipitata,Ulva intestinalis和Rhizoclonium sp.中生产的butanol进行比较分析. 使用Clostridium beijerinckii的ATCC 10132.2.使用了
- 塔古奇方法的应用,以优化低温水热预处理 (HTP) 条件的G. tenuistipitata.
- 预处理的大藻生物质的发酵,用于生产乙-butanol-乙醇 (ABE).
主要成果:
- 使用葡萄糖,Clostridium beijerinckii ATCC 10132 取得了14.07 g L-1 的高布塔诺度.
- 在测试的宏藻中,Gracilaria tenuistipitata表现出最高的butanol生产潜力 (1.38 g L-1).
- 优化HTP的G. tenuistipitata (110°C,10分钟,S/L 120,R01.29) 产生了最大的降解糖率为57.6%和ABE产量为19.87%.
- 低HTP (80°C,5分钟,S/L 50) 的G. tenuistipitata导致了3.1g L-1的布坦醇生产.
结论:
- 海洋大藻,特别是Gracilaria tenuistipitata,是通过ABE发酵生产生物butanol的可行的原料.
- 优化的低温水热预处理显著提高了从大藻中获得糖的产量和随后的布他诺产量.
- 这项研究强调了利用大藻类作为生物燃料开发的可持续和成本效益的第三代生物质的潜力.
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