相关实验视频
Updated: Jul 26, 2025

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
Published on: October 19, 2017
细胞细胞外陷的形成有助于宿主防御Clostridium perfringens感染
Yang Liu1, Jing Liang2, Jian-Wei Li2
1State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010070, China; Animal Husbandry Institute, Agriculture and Animal Husbandry Academy of Inner Mongolia, Hohhot 010031, China.
细胞细胞外陷 (ETs) 对于清除危险的病原体Clostridium perfringens至关重要. 防止外星人形成会增加对气体火和细菌殖民的易感性.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 杆菌会导致严重的感染,如气.
- 清除C. perfringens从宿主中的机制尚不清楚.
- 细胞细胞外陷 (ETs) 参与病原体清除.
研究的目的:
- 为了研究细胞ET在C. perfringens清除中的作用.
- 为了阐明C. perfringens诱导的ET形成的分子机制.
- 评估ET在C. perfringens感染中的治疗潜力.
主要方法:
- 用C. perfringens菌株刺激的巨细胞和中性粒细胞培养.
- 对ET组件的可视化 (DNA,基因组,MPO,NE).
- 使用途径抑制剂 (ERK1/2,P38 MAPK,SOCE,NADPH氧化酶) 和DNase I.的抑制研究.
- 在体内燃气瘤模型.
主要成果:
- 在巨细胞和中性粒细胞中,C. perfringens引发了显著的ET形成.
- ETs的形成取决于ERK1/2,P38 MAPK,SOCE,NADPH氧化酶,基因素,NE和MPO.
- 受损的外星人形成导致细菌杀死有缺陷.
- 在体内,ET的DNase I降解导致死亡率增加,组织损伤和细菌负载.
结论:
- 细胞ET的形成对于宿主防御C. perfringens.对抗至关重要.
- 外星人在控制气体热和减少细菌殖民方面发挥着至关重要的作用.
- 准ET代表了管理C. perfringens感染的潜在策略.
更多相关视频
14:05Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
Published on: November 4, 2022
08:08In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
Published on: November 1, 2019
相关概念视频
Immune Surveillance by NK Cells and Phagocytes
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytosis of Apoptotic Cells
Normal cells contain receptors that prevent them from being recognized...
Formation of Lipopolysaccharides
Adherens Junctions
Adherens Junctions are Dynamic
Phagocytosis
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells,...