前体和等离子体可以在它们携带的辅助基因类型中显示相反的趋势
Nobuto Takeuchi1,2, Sophia Hamada-Zhu1, Haruo Suzuki3,4
1School of Biological Sciences, the University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Proceedings. Biological sciences
|June 20, 2023
概括
菌体和质粒等移动遗传元素 (MGE) 携带辅助基因. 这项研究揭示了MGEs根据感染策略区分基因类型,如抗生素耐药性基因 (ARG) 和毒性因子基因 (VFG).
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 移动遗传元素 (MGE),包括菌体和质粒,通过携带辅助基因,为细菌进化做出贡献.
- 了解MGEs附属基因库的规则对于理解细菌适应至关重要.
研究的目的:
- 调查不同的MGEs,特别是prophages和plasmids,是否表现出携带抗生素耐药性基因 (ARGs) 和毒性因子基因 (VFGs) 的差异性模式.
- 根据MGEs的辅助基因含量,探索管理MGEs介导的水平基因转移的潜在规则.
主要方法:
- 在21种致病性细菌物种中对菌体和质粒基因组进行比较分析.
- 利用公共基因组数据库来评估MGE携带的ARG和VFG的频率.
- 检查了Escherichia coli*中由体和质粒编码的VFG的功能多样性.
主要成果:
- 在三种物种中,原体更频繁地携带VFG而不是ARG,而在九种物种中,等离子体更频繁地携带ARG而不是VFG.
- 在大肠杆菌*中,与等离子体传播的VFG相比,prophage编码的VFG具有更窄的功能范围 (宿主损伤,免疫调节).
- 在缺乏这种差异的物种中,ARG和VFG很少在prophages和plasmids中发现.
结论:
- MGEs在携带辅助基因方面表现出不同的模式,这表明与其特定的感染策略相关的规则.
- 基因内容的这种差异化支持了MGEs通过受规范的水平基因转移在塑造细菌进化中发挥作用的假设.
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