通过Xenopus蛋提取物抑制TGFβ和启动介质到上皮的过渡:迈向鱼体细胞早期重编程的第一步
Nathalie Chênais1, Aurelie Le Cam2, Brigitte Guillet3
1INRAE, UR1037 LPGP, Fish Physiology and Genomics, Campus de Beaulieu, 35000, Rennes, France. nathalie.chenais@inrae.fr.
Scientific reports
|June 20, 2023
概括
鱼蛋提取物在金鱼细胞中启动了部分介质细胞-上皮细胞过渡,这表明它可能会降低鱼类体质重编程的障碍. 需要进一步的研究才能充分理解重编程的潜力.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学 发展生物学
- 生殖生物学 生殖生物学
背景情况:
- 众所周知,Xenopus蛋提取物可以重编程培养的哺乳动物细胞.
- 了解它对非哺乳动物细胞的影响对于更广泛的应用至关重要.
研究的目的:
- 为了研究Xenopus蛋提取物对金鱼细胞的影响.
- 评估鱼类模型中细胞重编程的潜力.
主要方法:
- 金鱼细胞暴露于Xenopus卵取物在体外.
- 使用cDNA微阵列,基因本体学,KEGG通路和qPCR进行分析.
- 评估基因表达,甲基化和代谢变化.
主要成果:
- 抑制TGFβ和Wnt/β-catenin通路;上调上皮质标记物.
- 观察到介质细胞-上皮细胞过渡和细胞形态的改变.
- 部分重编程表明缺乏多能性标记物再表达和缺少促进物甲基化重塑.
结论:
- 鱼蛋提取物可以启动鱼细胞中的细胞变化,可能有助于重编程.
- 重编程是不完整的,在多能诱导和代谢转变方面存在局限性.
- 经过处理的细胞可以作为体内重编程研究的有价值模型.
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