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精油对牛乳腺炎中分离出来的金黄色葡萄球菌和葡萄球菌染色体的抗微生物活性
Tamiris Silva Lopes1, Caroline Fussieger1, Heloísa Theodoro2
1Diagnostic Laboratory of Veterinary Medicine, Biotechnology Institute, Universidade de Caxias Do Sul (UCS), Rua Francisco Getúlio Vargas 1130, Campus Sede, Caxias Do Sul, RS, 95070-560, Brazil.
概括
草和提精油对导致牛乳炎的葡萄球菌病原体具有强烈的抗菌活性. 这些植物衍生油因新出现的耐药性而为传统抗微生物药物提供了有希望的替代品.
科学领域:
- 兽医微生物学 兽医微生物学
- 植物化学 植物化学
- 自然产品化学 自然产品化学
背景情况:
- 牛乳炎是奶牛养殖业的重大经济问题,通常是由黄金葡萄球菌和染色体葡萄球菌引起.
- 细菌病原体抗微生物耐药性的增加需要探索替代治疗策略.
- 植物精油 (EO) 已因其潜在的抗菌特性而得到认可.
研究的目的:
- 评估来自五种不同的植物物种的精油对 Staphylococcus aureus 和 Staphylococcus chromogenes 的抗菌功效.
- 使用气相色谱 (GC) 确定选定的精油的化学成分.
- 评估EO的最小抑制度 (MIC) 和最小杀菌度 (MBC).
主要方法:
- 精油是通过水蒸从草,白,衣草,薄荷和西红中提取的.
- 气相色谱 (GC) 用于分析每个EO的化学成分.
- 进行了MIC和MBC测定,以量化抗菌活性对导致牛乳腺炎的葡萄球菌分离物.
主要成果:
- 草EO含有高水平的 (40.9%), (24.7%) 和 (1.9%).
- 红茶EO含有丰富的甲基醇 (44.2%),p-cymene (24.6%),以及1,8-cineole (9.9%).
- 草和西红花EOO表现出显著的抗菌活性,MIC和MBC值分别在0.39~3.12 mg/mL和0.39~6.35 mg/mL之间. 树,衣草和薄荷EOs没有显示出杀菌作用.
结论:
- 草和西红精油显示出作为天然抗菌剂的巨大潜力,可以对与牛乳腺炎有关的斯塔菲洛科克菌种进行抗菌.
- 这些发现支持使用草和西红EO作为常规抗微生物药物的可行替代品,用于控制牛乳腺炎,特别是考虑到抗微生物药物耐药性.
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