人类骨肌肉线粒体功能的侵入性和非侵入性标志物
Rodrigo Mancilla1,2,3, Diego Pava-Mejia1,2, Nynke van Polanen1,2
1NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, Maastricht, The Netherlands.
Physiological reports
|June 20, 2023
概括
评估骨肌肉的线粒体容量需要可靠的标记物. 复合V蛋白和酸盐合成酶活性 (侵入性),运动效率和PCr恢复 (非侵入性) 最好反映线粒体呼吸.
科学领域:
- 运动生理学 运动生理学
- 线粒体生物学 线粒体生物学
- 生物化学 生物化学
背景情况:
- 线粒体对于通过有氧新陈代谢来产生细胞能量至关重要.
- 准确评估骨肌肉的线粒体容量对于了解代谢健康和运动表现至关重要.
- 存在各种侵入性和非侵入性方法,但它们与线粒体呼吸的直接测量相关性需要澄清.
研究的目的:
- 评估骨肌肉线粒体容量的不同侵入性和非侵入性标记物之间的一致性,以及透气肌纤维中线粒体呼吸的直接测量.
- 为了确定评估线粒体呼吸能力的最可靠的替代品.
主要方法:
- 19名年轻男子接受了肌肉活检,以对线粒体呼吸,酸盐合成酶 (CS) 活性,线粒体DNA复制号,TOMM20,VDAC和氧化酸化 (OXPHOS) 蛋白质含量 (复合I-V) 进行侵入性评估.
- 非侵入性评估包括脂蛋白 (PCr) 恢复运动后 (通过31P-MRS),最大有氧容量 (VO2max) 和通过骑自行车的总体运动效率.
- 统计分析的重点是标记物和线粒体呼吸之间的一致性 (Rc).
主要成果:
- 侵入性标志物:复合V蛋白含量和CS活性与ADP刺激的合线粒体呼吸显示出最强的一致性 (Rc=0.500.72).
- 复合V蛋白含量显示出与最大分离线粒体呼吸的最高一致性 (Rc=0.72).
- 非侵入性标记:总体炼效率,VO2max和PCr恢复与ADP刺激的合呼吸显示一致 (Rc=0.500.77).
- 总体炼效率与最大不结合的呼吸有着最强的一致性 (Rc=0.67).
结论:
- 复合V蛋白含量和CS活性是骨肌肉线粒体呼吸能力的可靠侵入性替代品.
- 总体运动效率和运动后的PCr恢复是骨肌肉线粒体呼吸能力最准确的非侵入性指标.
相关概念视频
01:48Classification of Skeletal Muscle Fibers
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:10Integrins
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
01:17Connective Tissue Fibers and Ground Substance
One of the significant functions of connective tissue is connecting tissues and organs. Unlike epithelial tissue that is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. The matrix usually includes a large amount of extracellular material produced by the connective tissue cells that are embedded within it. It plays a significant role in the functioning of this tissue. The major component of the matrix is a...
01:26Bone Markings
Bones have various surface features that help form joints and attach to other soft tissues. Depending on the function, bone markings are categorized into articulating projections, processes for attachment, depressions, and openings.
Articulating Projections
Articulating projections are found where two bones meet to form a joint. These structures are usually found at the ends of bones. The largest articulation is a rounded projection called the head, supported by a narrow neck at the ends of...
Articulating Projections
Articulating projections are found where two bones meet to form a joint. These structures are usually found at the ends of bones. The largest articulation is a rounded projection called the head, supported by a narrow neck at the ends of...
00:55Fibrous Proteins
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...