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喷雾诱导的基因沉默用于识别粉状菌基因标和粉状菌控制过程

Amanda G McRae¹, Jyoti Taneja¹, Kathleen Yee¹

¹Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.

Molecular plant pathology

|June 21, 2023

概括

喷雾诱导基因沉默 (SIGS) 提供了一种新的方法来保护作物免受粉状真菌的侵害. 这种方法有效地减少了真菌基因表达,在不同的植物病原体系统中展示了广泛的适用性.

科学领域:

农业科学农业科学
植物病理学植物病理学
生物技术是生物技术.

背景情况:

粉菌在全球范围内造成重大作物损失.
粉状菌中抗真菌剂的耐药性需要新的控制策略.
喷雾诱导基因沉默 (SIGS) 是一种新兴的基于RNA干扰的技术,用于害虫控制.

研究的目的:

开发和优化SIGS用于控制粉状菌.
为了识别保存的基因标,对于粉状菌的扩散至关重要.
评估SIGS在不同病理系统中的疗效,包括葡萄粉.

主要方法:

优化了SIGS传递,针对Golovinomyces orontii-Arabidopsis thaliana*中的细胞染色体P450 51 (CYP51) 基因.
选了参与真菌新陈代谢,应激反应和宿主操纵的额外保存的基因标.
在 *Erysiphe necator-Vitis vinifera* (葡萄粉) 病理系统中测试了已识别的 SIGS 目标.

主要成果:

成功开发和优化SIGS用于粉状菌控制.
确定了保存的标,包括亡,脂质催化,酸代谢和效应体分泌中的标.
在使用SIGS的*A. thaliana*和*V. vinifera*系统中,已证明粉病的显著减少.

结论:

在模型病理系统中选保存目标有效地识别了控制其他粉状菌物种的目标.
SIGS是一种有前途且广泛适用的技术,用于商业粉状菌管理.
鉴定的基因标为开发耐药作物品种和有针对性的控制措施提供了新的途径.

关键词:

阿拉比多普西斯塔利亚纳.埃里西弗·内卡托尔 (Erysiphe necator) 是一个鱼.球类动物 (Golovinomyces orontii) 是一个类动物.dsRNARNA是什么意思葡萄藤是一种葡萄.粉状菌是一种粉状菌.喷雾诱导的基因沉默是一种喷雾诱导的基因沉默.

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