非侵入式检测,精确定位,并使用传输拉曼光谱在体内深层病变的术后导航使用传输拉曼光谱
Zongyu Wu1, Binge Deng1, Yutong Zhou1
1State Key Laboratory of Systems Medicine for Cancer, School of biomedical engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|June 21, 2023
概括
辐射测量表面增强传输拉曼光谱法 (SETRS) 能够非侵入性地精确地定位深层病变. 这种新的技术实时指导外科医生,改善了深部哨兵淋巴结检测和活检导航.
科学领域:
- 生物医学光学 生物医学光学
- 分子成像学分子成像学
- 手术导航 手术导航
背景情况:
- 光学技术提供高灵敏度,但在病变检测中难以透到深层组织.
- 准确的深度测定和深层病变的定位仍然是一个临床挑战.
- 传输拉曼光谱 (TRS) 是有前途的,但在体内应用中需要改进.
研究的目的:
- 开发和验证一个体内比率测量表面增强传输拉曼光谱 (SETRS) 系统.
- 为了实现深层病变的非侵入性检测,精确定位和外科导航.
- 为了克服光学成像模式中浅层组织透的局限性.
主要方法:
- 使用超明亮表面增强拉曼光谱 (SERS) 纳米粒子 (10 pM检测极限).
- 采用了自制的,光安全的传输拉曼光谱 (TRS) 设置.
- 开发了一种以几率计的SETRS策略,使用多个拉曼光谱峰的比率来确定深度.
主要成果:
- 在ex vivo老鼠组织中精确确定幻影病变深度,平均绝对百分比误差为11.8%.
- 已经准确地定位了6毫米深的老鼠尾淋巴结.
- 在安全的激光条件下,在活老鼠体内淋巴结活检的成功外科导航.
结论:
- 电比测量SETRS提供了一种可行的方法,用于深层病变的非侵入性局部化和深度评估.
- 开发的系统可为淋巴结活检等手术提供实时手术导航.
- 这一进步代表了向传输拉曼光谱的临床转化迈出的重要一步.
相关概念视频
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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01:20Raman Spectroscopy: Overview
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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