由Fragaria vesca在性繁殖期间经历的温度条件引起的甲基组,转录组和表型变化
Yupeng Zhang1,2, Tuomas Toivainen3, Kathryn Mackenzie3
1Department of Molecular Plant Biology, Norwegian Institute of Bioeconomy Research, Ås, Norway.
Physiologia plantarum
|June 21, 2023
概括
在不同的温度下种种,以影响特征. 森林草 (Fragaria vesca) 由于胚胎温度而显示持久的表型变化和DNA甲基化改变,揭示了表观遗传记忆效应.
科学领域:
- 植物科学 植物科学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 遗传学 是一个遗传学.
背景情况:
- 植物发育过程中的环境条件可以诱导表观遗传修饰.
- 这些表观遗传变化可以导致遗传的表型变异.
- 了解这些机制对于植物育种和适应至关重要.
研究的目的:
- 为了研究不同胚胎温度后森林草 (Fragaria vesca) 的持久表型效应和DNA甲基化变化.
- 为了确定温度诱导的表观遗传变化是否会产生类似记忆的反应.
- 探索生态型对温度变化的特定反应.
主要方法:
- 在胚胎形成和种子发育过程中,将五种欧洲生态型的Fragaria vesca种子暴露在两种温度 (18°C和28°C) 中.
- 在常见的花园条件下评估表型特征.
- 分析DNA甲基化模式和叶子转录组.
主要成果:
- 在从种子中培育的植物中观察到显著的表型差异,在三个四个特征中,在18°C和28°C下培育的种子中观察到显著的表型差异.
- 温度诱导的表观遗传记忆效应在两个生态型 (NOR2和ES12) 中显著,影响了诸如开花时间和生长点等特征.
- 在重复元素,伪基因和基因元素的生态型中发现了DNA甲基化在统计学上显著的差异,与生态型特定的转录基因变化.
结论:
- 胚胎温度可以诱导Fragaria vesca的长期表观遗传记忆类反应,影响表型特征.
- 生态型之间的遗传差异在这种表观遗传反应的可塑性中起作用.
- 治疗中DNA甲基化的变化表明胚胎发生过程中复杂的表观遗传重编程.
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