暴露于Pfaffia glomerata会引起氧化应激,并引发肝脏变化
F C R Dias1,2, M C Cupertino3,4, P G Silva5
1Universidade Federal do Triângulo Mineiro - UFTM, Departamento de Biologia Estrutural, Uberaba, MG, Brasil.
概括
在小鼠中引起肝损伤的Pfaffia glomerata四化水醇提取物. 这种药用植物提取物改变了氧化应激生物标志物,减少了活性的肝细胞,改变了矿物质水平,表明了潜在的毒性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 毒理学 毒理学 毒理学
- 植物化学 植物化学
背景情况:
- 药用植物是制药应用中二次代谢物的来源.
- 基因工程旨在增强药用植物中的生物质和代谢物生产.
- 这种植物名为Pfaffia glomerata (Spreng.) 佩德森是一种具有潜在制药用途的植物物种.
研究的目的:
- 为了评估Pfaffia glomerata四化水醇提取物在成年瑞士小鼠中的肝毒性作用.
- 评估提取物对肝脏氧化状态,矿物动力学和细胞活力的影响.
主要方法:
- 成年瑞士小鼠在42天的时间内通过 gavage 接受了Pfaffia glomerata四化水醇提取物 (100, 200, 400 mg/kg) 或不连续 (200 mg/kg) 的治疗.
- 对照组接受了饮用水.
- 分析肝脏氧化状态 (甲,氧化),矿物质含量 (铁,铜,,,,,),细胞活力和肝酶 (AST,ALT).
主要成果:
- 提取物减少了肝脏重量和可活性肝细胞的数量,尽管细胞数量总体增加.
- 高水平的甲和氧化表明氧化应激增加.
- 铁,铜,,,,和水平的显著变化被观察到.
- 酸氨基转移酶水平增加,而氨基转移酶水平下降.
结论:
- 在小鼠中,Pfaffia glomerata四化水醇提取物诱导了严重的肝损伤.
- 观察到的肝损伤与诱导的氧化应激和改变的矿物质平衡相关.
- 活跃肝细胞的减少表明了提取物的潜在毒性.
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