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维生素B12的参考时间间隔
Stine Linding Andersen1,2, Annebirthe Bo Hansen1, Peter Hindersson3
1Department of Clinical Biochemistry, Aalborg University Hospital.
Danish medical journal
|June 21, 2023
概括
不同的免疫试验给出了不可互换的血维生素B12结果和参考间隔. 诊断维生素B12缺乏症的临床指南必须考虑所采用的特定生化方法.
科学领域:
- 临床化学 临床化学
- 生物化学诊断 生物化学诊断
- 实验室医学 实验室医学
背景情况:
- 血维生素B12测试对于诊断缺乏症至关重要,但解释是复杂的.
- 对于维生素B12评估的各种生化方法的性能还没有很好地定义.
研究的目的:
- 用三种不同的免疫试验建立血维生素B12度参考间隔.
- 根据检测方法和切断值,评估低维生素B12度的频率.
主要方法:
- 血维生素B12度使用Alinity (Abbott),Cobas 6000 (Roche) 和Atellica IM (西门子) 免疫试验进行测量.
- 在献血者 (n=129) 中确定了直接参考间隔.
- 使用患者数据 (n=34,181) 建立了间接参考间隔,并分析了维生素B12 低水平的频率.
主要成果:
- 在这三种方法中,直接参考间隔在三种方法之间差异很大.
- 间接参考间隔也显示了免疫试验之间的显著差异.
- 维生素B12低于250 pmol/l的患者比例在14%至33%之间,取决于使用的方法.
结论:
- 血维生素B12测量及其参考间隔在不同的免疫测试中是不可互换的.
- 生物化学方法的选择显著影响维生素B12水平的解释.
- 临床指导方针应包括测试具体信息,以准确诊断维生素B12缺乏症.
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