斯基索奇菌种的发展. 株HS01具有高DHA和低和脂肪酸的生产,通过多端的适应性进化
Huichang Zhong1,2,3, Meng Zhang1, Liyi Chen4
1CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Biotechnology letters
|June 21, 2023
概括
实验室进化增强了精神分裂症患者的多可萨赫萨酸 (DHA) 生产. 改进的HS01菌株显示DHA含量较高,与中央代谢途径调节有关,而不是直接增强DHA生物合成.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 代谢工程是代谢工程.
背景情况:
- 多可萨赫萨酸 (DHA) 是一种重要的omega-3脂肪酸,在医学,食品和料中广泛应用.
- 微生物发酵,特别是使用Schizochytrium sp.,为DHA生产提供了一种高效和环保的方法.
- 应变改善对于优化DHA产量至关重要.
研究的目的:
- 为了提高Schizochytrium sp.的多可沙赫萨酸 (DHA) 生产性能. 使用实验室进化.
- 为了确定负责进化菌株中增强DHA生产的潜在分子机制.
主要方法:
- 应用了多方实验室进化 (ALE) 方法来开发一种高产量的DHA生产的精神分裂症菌株.
- 使用比较转录分析来比较进化菌株 (HS01) 与其父菌株 (GS00).
主要成果:
- 一个进化的菌株HS01呈现出更高的DHA含量和减少的和脂肪酸.
- 在HS01.01中,低的条件被确定为促进DHA生物合成的关键条件.
- 转录分析显示HS01中糖解,酸通路和TCA循环中的关键酶的上调调节,而DHA生物合成基因显示出与父菌株相似的表达水平.
结论:
- HS01菌株的增强的DHA生产能力归因于中央代谢途径的调节.
- 这项研究表明,优化中心代谢是提高DHA发酵产量的关键策略.
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