一个针对两个DNA区域的元编码协议,用于分析和白植物中的根相关的真菌群落
Thais Guillen-Otero1, Soon-Jae Lee2, Cheng-Wei Chen3
1Department of Systematic and Evolutionary Botany University of Zurich Zurich Switzerland.
Applications in plant sciences
|June 21, 2023
概括
这项研究引入了一种新的元编码协议,用于分析白植物和根中的真菌群落. ITS方法可靠地识别出一般的真菌群落,而18S rRNA的目标是状菌根真菌.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物科学 植物科学
- 进化生物学 进化生物学
背景情况:
- 菌类共生体对于陆地植物的进化至关重要.
- 以前的研究依赖于视觉根检查,限制了真菌社区分析.
- 元编码为研究植物和真菌相互作用提供了更全面的方法.
研究的目的:
- 建立和评估一个元编码协议的真菌群落在白植物和根.
- 为了比较ITS和18SrRNA测序对真菌社区分析的有效性.
- 调查地理位置对真菌根共生体的影响.
主要方法:
- 收集了来自12种不同的和白植物物种的根.
- 采用了两个针对ITS rRNA区域的原始对,用于一般的真菌查.
- 使用了18S rRNA原始蛋白来专门准Glomeromycota (状菌根真菌).
主要成果:
- 在ITS和18S rRNA数据集之间观察到显著的组成差异.
- ITS数据突出显示了Glomerales,Pleosporales和Helotiales的统治地位.
- 18S rRNA数据显示了更大的Glomeromycota多样性,对真菌群落产生地理影响.
结论:
- ITS元编码方法对于分析根和白植物根中的一般真菌群落是有效的.
- 18S rRNA方法更适合对状菌根真菌进行详细的查.
- 元编码协议增强了我们对早期植物-真菌进化动态的理解.
关键词:
在18S rRNA中.在DNA测序过程中,DNA测序它是它的ITS.这就是安普利康 (amplicon) 的意义.类的类 类的类这种植物是白植物 (lycophytes).超级编码元标段编码.菌根性真菌是一种菌根性真菌.更多相关视频
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