与怀孕相关的全身基因表达相比,与怀孕前的基线相比,在怀孕满期的健康女性中,与怀孕前的基线相比
Matthew L Wright1,2, Dana E Goin3, Mette Kiel Smed4
1Children's Hospital Oakland Research Institute, Oakland, CA, United States.
Frontiers in immunology
|June 21, 2023
概括
怀孕在健康女性中显著改变基因表达和细胞比例,免疫相关基因显示动态变化. 这些分子变化为了解妊娠并发症和自身免疫性疾病提供了基础.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 怀孕在母亲身上引发了显著的生物学变化,但分子水平的变化在很大程度上仍未被描述.
- 这项研究调查了在健康妇女怀孕期间和之后蛋白质编码基因和长非编码RNA的系统变化.
研究的目的:
- 综合分析健康怀孕中的分子和细胞变化.
- 为了确定正常怀孕的分子基线.
主要方法:
- 前性队列研究,采用7个时间点从14名健康妇女收集的血液样本.
- 全血的RNA测序,以量化蛋白质编码基因和长非编码RNA的基因表达.
- 用通用估计方程 (GEE) 模型分析基因表达的变化随着时间的推移,调整细胞类型的比例.
主要成果:
- 怀孕显著改变了许多免疫相关基因的表达,以时间依赖的方式.
- 与中性粒细胞相关的基因过度表达,而免疫球蛋白基因在怀孕期间表达不足.
- 细胞类型比例的变化,特别是中性粒细胞的增加,解释了大多数表达变化,但转录调节也起到了作用,特别是降低I型干扰素诱导基因的调节.
结论:
- 健康的怀孕与细胞比例和基因表达的广泛系统变化有关,部分是由于细胞转移,部分是由于转录调节.
- 这些发现提供了对正常怀孕的关键见解,并作为评估异常怀孕和与怀孕相关的自身免疫疾病的参考.
相关概念视频
Teratogenicity
2.5K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
2.5K
What is Gene Expression?
168.5K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
168.5K


