白细胞端粒长度和线粒体DNA拷贝数与与衰老相关的心血管疾病的内皮功能有关
Kangbo Li1,2, Mengjun Dai1,2, Mesud Sacirovic1
1Department for Angiology, Center for Internal Medicine I, Deutsches Angiologie Zentrum Brandenburg - Berlin, University Clinic Brandenburg, Brandenburg Medical School Theodor Fontane, Brandenburg an der Havel, Germany.
Frontiers in cardiovascular medicine
|June 21, 2023
概括
白细胞端粒长度 (TL) 和线粒体DNA复制数 (mtDNA-CN) 与心血管疾病 (CVD) 中的内皮功能相关. 白细胞TL和mtDNA-CN显示出作为内皮功能障碍的生物标志物的潜力.
科学领域:
- 生物化学 生物化学
- 遗传学 遗传学 是一个
- 心血管医学 心血管医学
背景情况:
- 与衰老相关的心血管疾病 (CVD) 是一个重大的健康问题.
- 内皮功能障碍是心血管疾病进展的关键因素.
- 端粒长度 (TL) 和线粒体DNA复制数 (mtDNA-CN) 是细胞衰老和压力的潜在指标.
研究的目的:
- 研究白细胞端粒长度 (TL) 与线粒体DNA复制数 (mtDNA-CN) 与心血管疾病患者内皮功能之间的关联.
- 探索无细胞DNA (cfDNA) 和白细胞DNA (leuDNA) 参数和内皮功能之间的关系.
- 在心血管疾病患者中确定内皮功能障碍的潜在生物标志物.
主要方法:
- 量化聚合酶链反应用于分析cfDNA和leuDNA中的相对端粒长度 (TL) 和线粒体DNA复制数 (mtDNA-CN).
- 通过测量流媒扩张 (FMD) 来评估内皮功能.
- 使用斯皮尔曼等级相关性和多重线性回归分析来检查变量之间的关联.
主要成果:
- 白细胞TL (leu-TL) 和白细胞mtDNA-CN (leu-mtDNA) 与内皮功能 (FMD) 有正相关性.
- 多重线性回归证实,leu-TL和leu-mtDNA与口病有积极的关联.
- 年龄与口病相反相关,表明内皮功能随着年龄的增长而下降.
结论:
- 端粒长度 (TL) 与线粒体DNA拷贝数 (mtDNA-CN) 在无细胞DNA (cfDNA) 和白细胞DNA (leuDNA) 中正相关.
- 白细胞端粒长度 (leu-TL) 和白细胞线粒体DNA复制数 (leu-mtDNA) 被确定为内皮功能障碍的潜在新生物标志物.
- 这些发现有助于理解心血管疾病中内皮功能障碍背后的分子机制.
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