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生物工艺开发的通用流感疫苗基于无活化分裂的奇美和马赛克血蛋白病毒

Eduard Puente-Massaguer¹, Annika Beyer¹, Madhumathi Loganathan¹

¹Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.

Frontiers in bioengineering and biotechnology

|June 21, 2023

概括

新的流感疫苗正在开发中,使用了嵌合体和马赛克血质素 (HA) 结构. 这项研究详细介绍了制造这些无活化分裂疫苗的生物工艺,旨在提供对季节性流感变种的更广泛保护.

科学领域:

病毒学病毒学
疫苗学疫苗学疫苗学
生物技术是生物技术.

背景情况:

每年季节性流感导致全球显著的发病率和死亡率.
目前针对血凝素 (HA) 和神经氨基酶 (NA) 的流感疫苗具有可变的有效性.
存在对疫苗的需求,以引起对保留的流感表位体更广泛的免疫力.

研究的目的:

开发一种生物工艺,用于制造无活化分裂疫苗,使用仿真HA (cHA) 和马赛克HA (mHA) 结构.
建立一种用预注液茎量化HA的方法.
优化疫苗生产以进行临床前和临床评估.

主要方法:

开发一种生物工艺,用于无活化分裂疫苗的生产.
使用β-propiolactone (βPL) 进行病毒无活化和Triton X-100进行分裂.
使用三明治酶链接免疫吸收试验 (ELISA) 量化输血前的HA.

主要成果:

优化的生物处理产生了大量的预注射HA和酶活性NA.
在最终的疫苗制剂中将剩余的Triton X-100和卵蛋白 (OVA) 降至最低水平.
证明成功制造cHA和mHA无活化分裂疫苗.

结论:

开发的生物工艺适合制造cHA和mHA无活化分裂疫苗.
这一过程为新型流感疫苗的临床前和临床研究提供了基础.
该方法可以适应生产针对其他流感病毒菌株的疫苗.

关键词:

哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈活动 NA 活动 NA.特里顿X-100可以使用.这是一种β-propiolactone.生物过程生物过程.在这里,我们可以看到CHAHA.mHAHA 在这里这是神经aminidase.

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