在用各种方法打开微管时,喷的速率
Henry L Wyneken1, Audrey A Cerles1, Kelly N Kim1
1Gryphon Scientific, Takoma Park, Maryland, USA.
概括
打开快门式微离心机管经常会导致喷,特别是在单手方法中. 这突显了实验室生物风险管理中的风险,以及需要采取安全预防措施的需要.
科学领域:
- 实验室的安全工作.
- 生物风险管理的管理
- 微生物学 微生物学
背景情况:
- 在生物实验室中,Snap-cap微离心管是常见的.
- 管开口时喷频率的数据是有限的.
- 了解喷频率对于有效的生物风险管理至关重要.
研究的目的:
- 在打开快门式微离心机管时量化喷频率.
- 为了在不同的打开方法中比较喷率.
- 评估表面和人员上的喷分布.
主要方法:
- 测试了四种不同的方法来打开快门管.
- 使用Glo Germ溶液作为标记器来检测喷.
- 测量了板,手套和实验室外套上的喷频率.
主要成果:
- 喷经常发生,无论使用的打开方法如何.
- 一只手的方法导致所有表面的喷率最高.
- 手套的喷率最高 (70-97%),其次是板 (2-40%).
结论:
- 所有经过测试的打开快门管的方法都经常产生喷.
- 一只手的方法是最容易出错的.
- 频繁的喷需要加强安全措施,如个人防护设备和二次封闭.
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