细胞收缩性驱动口腔状细胞癌的机械记忆
So Youn Moon1,2, Paloma Santos de Campos3, Bibiana Franzen Matte3
1Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Molecular biology of the cell
|June 21, 2023
概括
癌细胞可以"记住"硬的环境,通过机械记忆变得迁移. 这一过程涉及肌肉蛋白II,AKT和FAK信号,影响口腔状细胞癌 (OSCC) 的进展和患者的治疗结果.
科学领域:
- 在瘤学瘤学.
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
背景情况:
- 矩阵硬化在固体瘤中很常见,并驱动癌细胞迁移.
- 这种机制的机制和耐用性.
- 机械记忆 机械记忆是一种机械记忆.
- 在口腔平状细胞癌 (OSCC) 中的作用尚不清楚.
研究的目的:
- 调查OSCC中机械记忆获取和持久性背后的机制.
- 确定细胞收缩性和特定信号通路 (AKT,FAK) 在这种现象中的作用.
主要方法:
- 对比侵入性 (SSC25) 和非侵入性 (Cal27) OSCC细胞系.
- 将 Cal27 细胞暴露在刚性矩阵或收缩性激动剂中.
- 分析肌蛋白II的表达,表皮-介质细胞过渡 (EMT) 标志物和细胞迁移.
- 研究AKT和焦粘附激酶 (FAK) 信号传导的作用.
- 检查患者样本和转录基因数据.
主要成果:
- 长时间暴露在硬中诱导了Cal27细胞的迁徙表型,模仿侵入性细胞,在返回软基质 (机械记忆) 时持续存在.
- 这种表型的获取需要AKT信号,而在软基板上召回取决于FAK活动.
- 转录组分析揭示了与患者结果相关的持久差异.
结论:
- 细胞收缩性和下游信号通路 (AKT,FAK) 在OSCC中介于机械记忆.
- 这种记忆影响细胞表型和迁移,可能有助于瘤扩散.
- 研究结果强调了瘤微环境在癌症进展中的作用,并提出了治疗点.
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