KLF7通过激活PKCζ/NF-κB通路来促进脂肪细胞炎症和葡萄糖代谢障碍
Xin Yang1, Maodi Liang1, Yihan Tang1
1Department of Medical Genetics, Medical College of Shihezi University, Shihezi, China.
概括
转录因子KLF7通过调节蛋白激酶C zeta (PKCζ) 和激活核因子kappa B (NF-κB) 途径来促进与肥胖有关的炎症,从而增加脂肪细胞中的INTERLEUKIN-6 (IL-6) 表达.
科学领域:
- 代谢综合征是代谢综合征的一种.
- 脂肪细胞生物学 脂肪细胞生物学
- 分子内分泌学分子内分泌学
背景情况:
- 肥胖相关的炎症涉及脂肪细胞分泌的细胞因子,有助于胰岛素抵抗和代谢综合征.
- 之前的研究确定了KLF7 (克鲁佩尔类似因子7) 作为脂肪细胞中p-p65和IL-6的促进剂,但该机制尚未定义.
研究的目的:
- 阐明KLF7影响脂肪细胞炎性细胞因子表达的分子机制.
- 研究KLF7,蛋白激酶C zeta (PKCζ) 和核因子kappa B (NF-κB) 途径在脂肪细胞炎症中的作用.
主要方法:
- 在高脂肪饮食的小鼠和KLF7条件淘汰小鼠中分析KLF7,PKCζ,p-IκB,p-p65和IL-6表达.
- 使用3T3-L1脂肪细胞和HEK-293T细胞进行体外研究.
- 露西法酶记者测定和染色体免疫沉 (ChIP) 测定.
主要成果:
- 高脂肪饮食和KLF7增加了KLF7,PKCζ,p-IκB,p-p65和IL-6在皮膜白脂肪组织 (Epi WAT) 的表达.
- 在Epi WAT中,KLF7缺乏减少了PKCζ,p-IκB,p-p65和IL-6的表达.
- KLF7在转录水平上调节PKCζ,并通过脂肪细胞中的PKCζ/NF-κB通路促进IL-6的表达.
结论:
- KLF7通过上调PKCζ的调节来促进脂肪细胞中的IL-6表达.
- KLF7通过PKCζ激活NF-κB信号通路,导致脂肪细胞炎症.
- 这突出了一个关键机制,将KLF7与肥胖的代谢功能障碍联系起来.
关键词:
在IL-6的研究中,IL-6被认为是IL-6的基因.KLF7 KLF7 KLF7 KLF7 KLF7 KLF7 KLF7 KLF7 KLF7这就是NF-κBB.在PKCζζζ肥胖 肥胖 肥胖 肥胖 肥胖 肥胖 肥胖 肥胖更多相关视频
08:34Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.1K
09:41Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
2.9K
相关概念视频
02:26NF-κB-dependent Signaling Pathway
7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.5K
01:25cAMP-dependent Protein Kinase Pathways
6.4K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.4K
01:28Insulin: The Receptor and Signaling Pathways
1.3K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
1.3K
01:24Overview of Lipid Metabolism
1.8K
Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
1.8K
01:58Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
01:38Inflammation
54.0K
Overview
54.0K