内源RNAi的监管逻辑在沉默 de novo基因组冲突中发挥作用
Jeffrey Vedanayagam1, Ching-Jung Lin1,2, Ranjith Papareddy3
1Developmental Biology Program, Sloan Kettering Institute, New York, New York, United States of America.
PLoS genetics
|June 21, 2023
概括
在Drosophila中,内源性RNA干扰 (内-RNAi) 抑制了自私基因,防止了受损的精子生产. 在D. simulans中快速演变的毛RNAs (hpRNAs) 网络突出了它们在性染色体冲突和物种化中的作用.
科学领域:
- 遗传学 遗传学 是一个
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 内源RNA干扰 (内-RNAi) 功能在很大程度上仍未知.
- 自私的遗传元素可以破坏精子生成.
- 毛RNA (hpRNA) 位点产生siRNAs,这些siRNAs抑制了Drosophila中的介质驱动位点.
研究的目的:
- 研究内核RNAi在抑制Drosophila simulans中自私基因中的作用.
- 分析hpRNA网络及其目标的演变.
- 了解内分泌RNAi对基因表达和性染色体冲突的影响.
主要方法:
- 对Drosophila simulans和Drosophila melanogaster进行比较基因组分析.
- 对RNAi因子dcr-2的突变分析.
- 检查hpRNA-目标相互作用及其进化年龄.
主要成果:
- 与D. melanogaster相比,Drosophila simulans表现出最近出现的hpRNA-目标相互作用的显著扩展网络.
- 与Nmy/Dox相关的网络正在快速发展, hpRNAs经常针对丸的HMG盒位点.
- 最年轻的hpRNA目标显示出强烈的脱压力,而最老的目标显示出轻微的影响,表明了独特的监管影响.
结论:
- 在D. simulans中新的hpRNA调节网络提供了关于hpRNA出现和性染色体冲突的见解.
- 在内在性染色体冲突的早期阶段,内在RNAi至关重要.
- 由内核RNAi驱动的持续的基因扭曲和分辨周期可能会导致物种化.
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