通过便携式自动采集设备,每天高分辨率地绘制组织上腺类固醇的样本
Thomas J Upton1, Eder Zavala2, Paal Methlie3,4
1Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, Translational Health Sciences, Faculty of Health Sciences, University of Bristol, Bristol BS1 3NY, UK.
Science translational medicine
|June 21, 2023
概括
被破坏的上腺激素节律是早期疾病的信号. 这项研究使用微透析在健康个体中创建组织上腺类固醇的高分辨率24小时概况,建立正常性的动态标记.
科学领域:
- 内分泌学 在内分泌学.
- 时间生物学 时间生物学
- 生物标志物发现发现
背景情况:
- 内分泌系统依赖于荷尔蒙波动;干扰是早期疾病指标.
- 循环和超定时上腺激素分泌模式限制了来自单次时间点测量的信息.
- 为了监测荷尔蒙,在夜间采取血液样本是侵入性的,并且会扰乱睡眠.
研究的目的:
- 开发一种微创方法,在24小时内高分辨率地对组织中的自由上腺类固醇进行分析.
- 克服传统血液采样的局限性,以评估激素节律性,特别是在睡眠期间.
- 在健康个体中建立上腺类固醇水平的规范性动态标记.
主要方法:
- 使用微透析和门诊分数采集器进行持续24小时的组织采样.
- 采用液体染色学-并联质谱法 (LC-MS/MS) 精确量化上腺类固醇.
- 验证的组织测量与参与者的子集中的血度相比.
主要成果:
- 证明了门诊微透析用于皮下组织采样的安全性和耐受性.
- 在多种自由上腺类固醇中确定了每日和超日变化,包括皮质醇,皮质素和阿尔多斯特.
- 量化了个体间的变异性,并开发了性别,年龄和BMI分层的动态参考范围.
结论:
- 微透析提供高分辨率的组织激素配置文件,提供了对实际环境中的上腺类固醇动态的洞察力.
- 这种方法克服了评估激素节律性的传统血液采样的局限性.
- 已建立的动态标记可以作为诊断内分泌疾病的潜在规范性参考.
相关概念视频
01:31Hormones of the Adrenal Glands
2.6K
Adrenal hormones play a pivotal role in maintaining the body's electrolyte balance and orchestrating responses to stress, showcasing the intricate functions of the adrenal cortex and medulla.
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
2.6K
01:17Anatomy of the Adrenal Glands
2.4K
The adrenal or supra-renal glands, situated above the kidneys and aligned with the twelfth rib, are paired pyramid-shaped structures crucial for the body's stress response. During stress, these glands secrete hormones vital for adaptive physiological reactions.
These glands possess a distinctive yellow tinge due to the stored cholesterol and fatty acids required for hormone synthesis. They are encased in a fibrous capsule and cushioned by fat.
The adrenal gland comprises two distinct...
These glands possess a distinctive yellow tinge due to the stored cholesterol and fatty acids required for hormone synthesis. They are encased in a fibrous capsule and cushioned by fat.
The adrenal gland comprises two distinct...
2.4K
01:27Adrenal Gland Disorders
1.6K
Adrenal gland disorders manifest when the production of adrenal hormones deviates from the norm, resulting in either excessive or insufficient concentrations.
Adrenal insufficiency, characterized by insufficient cortisol and aldosterone production, leads to conditions like Addison's disease. This disorder, affecting the adrenal cortex, exhibits symptoms such as skin bronzing, dehydration, low blood pressure, fatigue, and weight loss. Congenital adrenal hyperplasia, a genetic ailment causing...
Adrenal insufficiency, characterized by insufficient cortisol and aldosterone production, leads to conditions like Addison's disease. This disorder, affecting the adrenal cortex, exhibits symptoms such as skin bronzing, dehydration, low blood pressure, fatigue, and weight loss. Congenital adrenal hyperplasia, a genetic ailment causing...
1.6K