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乌贝洛普西斯 (Umbelopsis ramannianaana) 优化了胡卜素的产生

Hatice Kübra Kizilay¹, Ahmet Küçükçetin¹, Muammer Demir¹

¹Faculty of Engineering, Department of Food Engineering, Akdeniz University, Antalya, Turkey.

Biotechnology progress

|June 21, 2023

概括

这项研究优化了Umbelopsis ramanniana,使其产生更多的胡卜素和生物质. 通过使用乳糖和KNO3,研究人员通过先进的统计设计实现了显著的产量增加.

科学领域:

微生物学微生物学
生物技术是生物技术.
生物化学生物化学

背景情况:

胡卜素是具有显著健康益处的重要颜料.
乌姆贝洛普西斯 (Umbelopsis ramanniana) 是一种具有类生物合成潜力的真菌物种.
优化发酵条件对于最大限度地提高微生物产品产量至关重要.

研究的目的:

为了增强Umbelopsis ramanniana中的胡卜素和生物质生产.
为了确定最佳的碳和来源,用于真菌生长和颜料合成.
确定理想的介质组成和发酵参数,以获得最大的产量.

主要方法:

对9个碳源和6个源进行选,以确定最佳营养素.
应用Plackett-Burman设计用于初始中介组件的优化.
使用Box-Behnken响应表面方法来微调发酵参数.
研究乳糖度的影响,碳与的比率,以及振动速度.

主要成果:

酸 (KNO3) 和乳糖分别被确定为最有效的和碳来源.
优化条件包括32.42g/L的乳糖, 20:1的碳比,以及130rpm的速度.
在胡卜素中达到1141μg/L (β-胡卜素-Eq) 的最大产量,在生物质中达到13.14g/L.
与对照组相比,胡卜素和生物质生产增加了约2倍和1.3倍.

结论:

这项研究成功地优化了Umbelopsis ramanniana发酵,提高了胡卜素和生物质的生产.
确定的最佳条件为工业规模生产真菌类胡卜素提供了坚实的框架.
进一步的研究可以探索基因修饰,以进一步促进胡卜素生物合成途径.

关键词:

盒子-Behnken设计的设计.普莱克特-伯曼设计乌贝洛普西斯拉曼尼亚拉曼尼亚拉曼尼亚胡卜素是一种胡卜素.响应表面方法响应表面方法

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