bZIP23与NAC028相互作用,调节大米对病的抗性
De Peng Yuan1, Zhuo Li2, Huan Chen2
1Rice Research Institute, Shenyang Agricultural University, Shenyang, 110866, China; College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Biochemical and biophysical research communications
|June 21, 2023
概括
抗米病耐药性涉及NAC028和bZIP23的转录因子. 这些通过CAD8B调节素生物合成,增强植物对疾病的防御能力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 米病 (ShB) 对全球大米产量构成重大威胁.
- 开发耐ShB米品种对于作物保护至关重要.
- 导致大米ShB耐药性的分子机制在很大程度上仍未被探索.
研究的目的:
- 调查NAC转录因子NAC028在大米ShB耐药性中的作用.
- 阐明参与ShB防御的分子相互作用和调节途径.
- 确定在米育种计划中增强ShB耐药性的潜在目标.
主要方法:
- 在大米植物中进行ShB接种试验.
- 使用酵母二混合试验识别相互作用蛋白质.
- 转录组和定量实时PCR (qRT-PCR) 分析.
- 酵母-一种混合,ChIP-qPCR和交换活化试验.
- 在体外和体外基因表达分析.
主要成果:
- NAC028 作为 ShB 抗米的积极调节剂.
- 转录因子bZIP23与NAC028.8相互作用.
- NAC028和bZIP23都直接结合CAD8B的促进子,这是一个关键的素生物合成基因,并激活其表达.
- NAC028是bZIP23的目标基因,显示出一个调控层次结构.
结论:
- NAC028和bZIP23形成了一个调节复合体,增强了ShB的抗性.
- 这种bZIP23-NAC028-CAD8B通路对于防治囊病的防治至关重要.
- 这些发现为开发抗ShB的米品种提供了有价值的分子见解.
相关概念视频
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:09Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...