模拟医院消毒剂对在人造汗水下生长的多药耐药干表面生物膜
F Watson1, S Wilks2, C W Keevil3
1School of Biological Sciences, University of Southampton, Southampton, UK; Bioquell UK Ltd, Andover, UK.
The Journal of hospital infection
|June 21, 2023
概括
人工汗水中的干表面生物膜 (DSB) 显示出比常规介质中的抗微生物耐受性更高. 当前的消毒剂测试标准可能高估了清洁剂对抗抗性医院病原体的有效性.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 环境科学 环境科学
背景情况:
- 医院表面的干表面生物膜 (DSB) 有助于医院病原体的持续存在.
- 目前的消毒剂测试使用浮游生物模型,它们的耐受性低于生物膜.
研究的目的:
- 评估在人造汗水 (AHS) 中培养的生物膜模型,以评估抗菌性能.
- 为了比较在AHS中生长的生物膜对消毒剂的有效性与富含营养的介质.
主要方法:
- 在不钢上使用致病细菌生成了五种单个物种的生物膜.
- 在AHS和营养丰富的条件下化生物膜,然后进行干燥.
- 暴露于常见消毒剂的生物膜,并使用光显微镜评估生存能力.
主要成果:
- 与常规介质相比,AHS中的生物膜显示出显著更高的抗微生物耐受性和更慢的杀死率.
- 在消毒后检测到有活力的细菌,这表明有可能重新生长.
结论:
- 人工汗水 (AHS) 更好地模仿医院表面的营养条件,从而产生更有弹性的生物膜.
- 当前的抗微生物测试标准可能无法准确地反映表面上的真实微生物挑战.
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