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High-throughput Saccharification Assay for Lignocellulosic Materials
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通过高效的组合预处理,改善了布鲁什的酶糖化
Ying Chen1, Dong Yang1, Wei Tang1
1School of Pharmacy & School of Biological and Food Engineering, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou 213164, PR China.
Bioresource technology
|June 21, 2023
概括
这项研究表明,将深层水溶剂 (DES) 与氧化 (NaOH) 结合起来,可以有效地从草生物质中去除红素和西兰. 这种预处理显著提高了纤维素的酶消化能力和糖化活性.
科学领域:
- 生物质预处理 生物质预处理
- 绿色化学 绿色化学
- 生物炼油工艺 生物炼油工艺
背景情况:
- 有效的预处理对于从纤维素纤维素生物质中释放纤维素至关重要.
- 深层欧特基溶剂 (DES) 为生物质加工提供了一个可持续的替代方案.
- 布鲁什为生物燃料和生物化学生产提供了潜在的原料.
研究的目的:
- 为了研究胆化物:甘油 (ChCl:Gly) DES和NaOH对冲压预处理的联合作用.
- 评估这种联合预处理对生物质组成,微观结构和酶化水解的影响.
- 优化基于DES的预处理,以提高纤维素的可访问性和糖化.
主要方法:
- 在60分钟的时间内,在100°C使用Cl:Gly (20%重量) 和NaOH (4%重量) 混合物进行了预处理.
- 分析包括化学成分 (木质素,西兰,纤维素),微观结构,晶体结构和细胞酶水解.
- 测量了一些关键参数,如生物质可访问性,疏水性和素表面积.
主要成果:
- 综合NaOH-ChCl:Gly预处理中,去除了80.1%的红素和66.8%的西兰.
- 纤维素的酶性消化率达到87.9%,可获得性增加到645.2 mg/g.
- 纤维素结晶性从20.8%增加到55.6%,降低了疏水性和木质素表面积.
结论:
- 结合DES和的预处理是非常有效的delignification和bulrush的de-xylanization.
- 增强的生物质结构促进了显著改善的纤维素酶化水解和糖化.
- 这种方法提供了一个有前途的战略,用于有效地利用生物炼油厂中的草.
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