用聚乳酸堆肥食品废弃物的抑制性能:强调微生物核心代谢途径和机制
Yi Zheng1, Ziwei Feng2, Pan Wang3
1College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Suzhou 215128, China.
Bioresource technology
|June 21, 2023
概括
食品废弃物 (FW) 堆肥中的多乳酸 (PLA) 降低了微生物多样性,并改变了关键的代谢途径. 此外,PLA堆肥还影响了三碳酸循环,降低了发芽率.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废物管理 废物管理
背景情况:
- 食品废弃物 (FW) 堆肥是一种可持续的废物处理方法.
- 聚乳酸 (PLA) 是一种可生物降解的聚合物,越来越多地用于包装.
- 了解PLA对FW堆肥的影响对于优化该过程至关重要.
研究的目的:
- 调查PLA在FW堆肥过程中对微生物群落结构的影响.
- 分析PLA对堆肥食品废物的核心代谢途径的影响.
- 阐明控制FW和PLA共同堆肥的基因水平机制.
主要方法:
- 进行了对食品废弃物添加PLA和没有PLA的堆肥实验.
- 用分子技术评估微生物群体的组成.
- 通过检查基因表达进行了代谢途径分析.
- 用发芽指数来评估堆肥成熟度和植物毒性.
主要成果:
- 在不同堆肥阶段,PLA的存在对微生物丰富性和关键细菌属 (Bacillus,Halocella,Cellvibrio) 的丰富性产生了负面影响.
- 在热友和成熟阶段,PLA降解刺激了乳酸代谢基因的表达.
- 参与三碳酸循环的碳氧化作用的功能基因的丰富性随着PLA的添加而减少.
- 在热友性阶段,干扰2-Oxoglutarate转化为Succinyl-CoA的过程与PLA组中较低的发芽指数相关.
结论:
- 在食品废弃物堆肥过程中,PLA显著改变了微生物动力学和代谢功能.
- 三碳酸循环和随后的堆肥质量,以发芽指数表示,受到PLA联合堆肥的负面影响.
- 基因表达分析提供了对PLA在堆肥系统中的作用背后的生化机制的见解.
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