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改变了SARS-CoV-2尖端蛋白突变体的 hACE2结合亲和力和S1/S2裂解效率,影响病毒细胞进入

Ke Wang¹, Yu Pan², Dianbing Wang²

¹National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Faculty of Synthetic Biology, Shenzhen Institute of Advanced Technology, Shenzhen, 518055, China; University of Chinese Academy of Sciences, Beijing, 100101, China.

Virologica Sinica

|June 21, 2023

概括

新的SARS-CoV-2变种在细胞进入上表现出不同的尖端蛋白效应. 这就是Omicron.

科学领域:

病毒学病毒学
分子生物学分子生物学
流行病学流行病学

背景情况:

新出现的SARS-CoV-2变种对感染控制构成挑战.
尖端蛋白突变影响病毒的进入,需要进行详细的比较.

研究的目的:

综合分析尖端蛋白突变对SARS-CoV-2变种的影响.
为了比较变体的尖峰表达,结合,进入效率和宿主细胞热带性.

主要方法:

研究了八种令人担忧 (VOC) 和感兴趣 (VOI) 的变体.
评估了使用伪病毒的尖端蛋白表达,蛋白质分解活性,与 hACE2 的结合亲和力,以及病毒进入效率.
研究了宿主细胞的热带性和TMPRSS2.2.的利用.

主要成果:

欧米克朗尖峰和RBD与hACE2的结合亲和力类似于野生类型.
阿尔法,贝塔,德尔塔和兰巴达伪病毒显示增强的细胞进入;Omicron显示轻微增加.
奥米克朗和Mu尖峰显示较低的S1/S2裂变和低效的TMPRSS2使用,表明细胞内的偏好.
所有变体都可能进入动物ACE2表达细胞,在动物中增加感染潜力,这表明病毒进化的潜在储库.

结论:

奥米克朗的高传播率与hACE2亲和关系无关,但可能是其他进入途径.
动物可能会成为SARS-CoV-2进化的储备体,因为它们的易感性增加了.
这些发现指导了流行病控制策略,并增强了对SARS-CoV-2演变的理解.

关键词:

结合性亲缘关系是一种结合性亲缘关系.主体热带主义是一个主体.这就是Omicron.这就是SARS-CoV-2病毒.关注的变种 (VOC)病毒的入口病毒的入口

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